mRNA expression and DNA methylation in three key genes involved in caste differentiation in female honeybees (Apis mellifera)
文献类型：期刊论文
| 作者 | Shao XL1,2,3; He SY1,3,4; Zhuang XY2; Fan Y1,3; Li YH[*]1,3,4; Yao YG2 |
| 刊名 | Zoological Research
 |
| 出版日期 | 2014 |
| 卷号 | 35期号:2页码:92−98 |
| 关键词 | Caste differentiation DNA methylation DNA methyltransferase 3 Honeybee Target of rapamycin |
| 通讯作者 | liyh85@aliyun.com |
| 合作状况 | 其它 |
| 英文摘要 | In honeybee (Apis mellifera) colonies, queens and workers are alternative forms of the adult female honeybee that develop from genetically identical zygotes but that depend on differential nourishment. Queens and workers display distinct morphologies, anatomies and behavior, better known as caste differentiation. Despite some basic insights, the exact mechanism responsible for this phenomenon, especially at the molecular level, remains unclear although some progress has been achieved. In this study, we examined mRNA levels of the TOR (target of rapamycin) and Dnmt3 (DNA methyltransferase 3) genes, closely related to caste differentiation in honeybees. We also investigated mRNA expression of the S6K (similar to RPS6-p70-protein kinase) gene linked closely to organismal growth and development in queen and worker larvae (1-day and 3-day old). Last, we investigated the methylation status of these three genes in corresponding castes. We found no difference in mRNA expression for the three genes between 1st instar queen and worker larvae; however, 3rd instar queen larvae had a higher level of TOR mRNA than worker larvae. Methylation levels of all three genes were lower in queen larvae than worker larvae but the differences were not statistically significant. These findings provide basic data for broadening our understanding of caste differentiation in female honeybees. |
| 收录类别 | 其他 |
| 资助信息 | This work was supported by the Yunnan Gover- nment (2009CI119) and Modern Agricultural Industry Technology System (Honeybee) (CARS-45-kxj14) |
| 语种 | 英语 |
| 公开日期 | 2014-03-19 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7820]  |
| 专题 | 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming 650223, China 3.Eastern Bee Institute, Yunnan Agricultural University, Kunming 650201, China; 4.College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China |
| 推荐引用方式 GB/T 7714 | Shao XL,He SY,Zhuang XY,et al. mRNA expression and DNA methylation in three key genes involved in caste differentiation in female honeybees (Apis mellifera)[J]. Zoological Research,2014,35(2):92−98. |
| APA | Shao XL,He SY,Zhuang XY,Fan Y,Li YH[*],&Yao YG.(2014).mRNA expression and DNA methylation in three key genes involved in caste differentiation in female honeybees (Apis mellifera).Zoological Research,35(2),92−98. |
| MLA | Shao XL,et al."mRNA expression and DNA methylation in three key genes involved in caste differentiation in female honeybees (Apis mellifera)".Zoological Research 35.2(2014):92−98. |
入库方式： OAI收割
来源：昆明动物研究所
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