Genetic variations of mitochondrial antiviral signaling gene (MAVS) in domestic chickens
文献类型：期刊论文
| 作者 | Yu DD1; Xu L1,2; Peng L1,2; Chen SY3; Liu YP3,4; Yao YG[*]1 |
| 刊名 | GENE
 |
| 出版日期 | 2014 |
| 卷号 | 545期号:2页码:226-232 |
| 关键词 | 3'-UTR Chicken Haplotype MAVS Polymorphism |
| 通讯作者 | ygyaozh@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Mitochondrial antiviral signaling (MAVS) gene plays a key role in antiviral regulation in mammals potentially by activating IRF3/7 and NF-kappa B and leading to the induction of type I interferon (IFN)-mediated antiviral and inflammatory responses. In this study, we screened genetic polymorphisms of the MAVS gene in various Chinese domestic chicken breeds/populations and evaluated its potential effect on gene expression. Among the sequenced fragment (4678 bp), a total of 75 single nucleotide polymorphisms (SNPs) were identified in 46 chickens from 10 breeds/populations, including 30 coding SNPs and 45 non-coding SNPs. Extremely high haplotype diversity (37 nucleotide haplotypes, 18 amino add haplotypes) was observed in the coding region (CDS), and a similar pattern of high polymorphisms was also observed for the 3'-untranslated region (3'-UTR). Luciferase assays of two representative 3'-UTR haplotypes were performed in both HEK293 cells and DF-1 chicken fibroblast cells, and we found that they were differentially associated with different abilities on regulating mRNA expression level (P < 0.05). Collectively, we observed a considerably high genetic variability of the MAVS gene, and the 3'-UTR variants had an ability to regulate mRNA expression. These results would cast some clues on understanding the potential role of MAVS on viral resistance in chicken. |
| 资助信息 | This study was sup- ported by the National Science Foundation of China (31001006) and Yunnan Province (2008CD175). |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000337867400007 |
| 公开日期 | 2014-06-06 |
| 源URL | [http://159.226.149.42:8088/handle/152453/7896]  |
| 专题 | 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China 3.Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu Campus, Chengdu 611130, China 4.College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, China |
| 推荐引用方式 GB/T 7714 | Yu DD,Xu L,Peng L,et al. Genetic variations of mitochondrial antiviral signaling gene (MAVS) in domestic chickens[J]. GENE,2014,545(2):226-232. |
| APA | Yu DD,Xu L,Peng L,Chen SY,Liu YP,&Yao YG[*].(2014).Genetic variations of mitochondrial antiviral signaling gene (MAVS) in domestic chickens.GENE,545(2),226-232. |
| MLA | Yu DD,et al."Genetic variations of mitochondrial antiviral signaling gene (MAVS) in domestic chickens".GENE 545.2(2014):226-232. |
入库方式： OAI收割
来源：昆明动物研究所
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