Mutation and expression analysis of the IDH1, IDH2, DNMT3A, and MYD88 genes in colorectal cancer
文献类型：期刊论文
| 作者 | Li WL[*]1; Xiao MS2; Zhang DF2,5; Yu DD2; Yang RX3; Li XY4; Yao YG[*]2,5 |
| 刊名 | GENE
 |
| 出版日期 | 2014 |
| 卷号 | 546期号:2页码:263-270 |
| 关键词 | Mutation Expression IDH1 IDH2 DNMT3A MYD88 |
| 通讯作者 | wl.li@aliyun.com ; ygyaozh@gmail.com |
| 合作状况 | 其它 |
| 英文摘要 | Colorectal cancer (CRC) is one of the leading causes of death around the world. Its genetic mechanism was intensively investigated in the past decades with findings of a number of canonical oncogenes and tumor-suppressor genes such as APC, KRAS, and TP53. Recent genome-wide association and sequencing studies have identified a series of promising oncogenes including IDH1,IDH2, DNMT3A, and MYD88 in hematologic malignancies. However, whether these genes are involved in CRC remains unknown. In this study, we screened the hotspot mutations of these four genes in 305 CRC samples from Han Chinese by direct sequencing. mRNA expression levels of these genes were quantified by quantitative real-time PCR (RT-qPCR) in paired cancerous and paracancerous tissues. Association analyses between mRNA expression levels and different cancerous stages were performed. Except for one patient harboring IDH1 mutation p.I99M, we identified no previously reported hotspot mutations in colorectal cancer tissues. mRNA expression levels of IDH1, DNMT3A, and MYD88, but not IDH2, were significantly decreased in the cancerous tissues comparing with the paired paracancerous normal tissues. Taken together, the hotspot mutations of IDH1, IDH2, DNMT3A, and MYD88 gene were absent in CRC. Aberrant mRNA expression of IDH1, DNMT3A, and MYD88 gene might be actively involved in the development of CRC. |
| 收录类别 | SCI |
| 资助信息 | This study was supported by Chinese Academy of Sciences and Yunnan Province (2009CI119). |
| 语种 | 英语 |
| WOS记录号 | WOS:000339150800019 |
| 公开日期 | 2014-10-21 |
| 源URL | [http://159.226.149.42:8088/handle/152453/8112]  |
| 专题 | 昆明动物研究所_重大疾病机理的遗传学 昆明动物研究所_动物模型与人类重大疾病机理重点实验室 |
| 作者单位 | 1.Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China 2.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, Yunnan 650223, China 3.Chemotherapy Research Center, Yunnan Provincial Tumor Hospital, Kunming Medical University, Kunming, China 4.Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, China 5.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China |
| 推荐引用方式 GB/T 7714 | Li WL[*],Xiao MS,Zhang DF,et al. Mutation and expression analysis of the IDH1, IDH2, DNMT3A, and MYD88 genes in colorectal cancer[J]. GENE,2014,546(2):263-270. |
| APA | Li WL[*].,Xiao MS.,Zhang DF.,Yu DD.,Yang RX.,...&Yao YG[*].(2014).Mutation and expression analysis of the IDH1, IDH2, DNMT3A, and MYD88 genes in colorectal cancer.GENE,546(2),263-270. |
| MLA | Li WL[*],et al."Mutation and expression analysis of the IDH1, IDH2, DNMT3A, and MYD88 genes in colorectal cancer".GENE 546.2(2014):263-270. |
入库方式： OAI收割
来源：昆明动物研究所
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