Multivalent Chromatin Engagement and Inter-domain Crosstalk Regulate MORC3 ATPase
文献类型：期刊论文
| 作者 | Andrews, Forest H.1; Tong, Qiong1,5; Sullivan, Kelly D.1,4; Cornett, Evan M.2; Zhang, Yi1; Ali, Muzaffar1; Ahn, JaeWoo1; Pandey, Ahway1,4; Guo, Angela H.3; Strahl, Brian D.3 |
| 刊名 | CELL REPORTS
 |
| 出版日期 | 2016-09-20 |
| 卷号 | 16期号:12页码:3195-3207 |
| 英文摘要 | MORC3 is linked to inflammatory myopathies and cancer; however, the precise role of MORC3 in normal cell physiology and disease remains poorly understood. Here, we present detailed genetic, biochemical, and structural analyses of MORC3. We demonstrate that MORC3 is significantly upregulated in Down syndrome and that genetic abnormalities in MORC3 are associated with cancer. The CW domain of MORC3 binds to the methylated histone H3K4 tail, and this interaction is essential for recruitment of MORC3 to chromatin and accumulation in nuclear bodies. We show that MORC3 possesses intrinsic ATPase activity that requires DNA, but it is negatively regulated by the CW domain, which interacts with the ATPase domain. Natively linked CW impedes binding of the ATPase domain to DNA, resulting in a decrease in the DNA-stimulated enzymatic activity. Collectively, our studies provide a molecular framework detailing MORC3 functions and suggest that its modulation may contribute to human disease. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Cell Biology |
| 研究领域[WOS] | Cell Biology |
| 关键词[WOS] | STRUCTURAL INSIGHT ; PROTEIN NXP-2 ; CW DOMAIN ; HISTONE ; BINDING ; FAMILY ; PHD ; DERMATOMYOSITIS ; AUTOANTIBODIES ; TRANSCRIPTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000383884200012 |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/9615]  |
| 专题 | 武汉物理与数学研究所_磁共振应用研究部 |
| 作者单位 | 1.Univ Colorado, Sch Med, Dept Pharmacol, Aurora, CO 80045 USA 2.Van Andel Res Inst, Ctr Epigenet, Grand Rapids, MI 49503 USA 3.Univ N Carolina, Sch Med, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA 4.Univ Colorado, Sch Med, Linda Crnic Inst Syndrome, Aurora, CO 80045 USA 5.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Andrews, Forest H.,Tong, Qiong,Sullivan, Kelly D.,et al. Multivalent Chromatin Engagement and Inter-domain Crosstalk Regulate MORC3 ATPase[J]. CELL REPORTS,2016,16(12):3195-3207. |
| APA | Andrews, Forest H..,Tong, Qiong.,Sullivan, Kelly D..,Cornett, Evan M..,Zhang, Yi.,...&Kutateladze, Tatiana G..(2016).Multivalent Chromatin Engagement and Inter-domain Crosstalk Regulate MORC3 ATPase.CELL REPORTS,16(12),3195-3207. |
| MLA | Andrews, Forest H.,et al."Multivalent Chromatin Engagement and Inter-domain Crosstalk Regulate MORC3 ATPase".CELL REPORTS 16.12(2016):3195-3207. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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