A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography
文献类型：期刊论文
| 作者 | Chen, Quan1; Latiff, Sarah Maria Abdul2; Toh, Phyllicia2; Peng, Xinying1; Hoi, Aina2; Xian, Mo1 ; Zhang, Haibo1; Nian, Rui1; Zhang, Wei2; Gagnon, Pete2
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| 刊名 | JOURNAL OF BIOTECHNOLOGY
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| 出版日期 | 2016-10-20 |
| 卷号 | 236页码:128-140 |
| 关键词 | Precipitation Monoclonal antibody Purification Chromatin-directed clarification Light chain Surfactant |
| 英文摘要 | Protein A affinity chromatography, featured by its robustness and high-specificity, is still dominant as a first capture step for the purification of immunoglobulin G monoclonal antibodies (IgG mAbs). However, the material and operational costs of protein A are universally recognized as high, and its productivity is also limited as column mode. In order to overcome these limitations, industry is increasingly considering the use of non-protein A-based processes for IgG purification. In this study, sodium citrate precipitation (SCP) was developed as the primary purification step, and chromatin-directed cell culture clarification was demonstrated to significantly elevate the purification capability. Additional 0.05% (w/v) of Tween 20 was shown to effectively reduce the residual free antibody light chain (LC) during precipitation. The resuspended IgG was further polished by void-exclusion anion exchange chromatography (VEAX), which supported protein loading without buffer adjustment. The non-histone host cell protein (nh-HCP) content in the final product was about 5 ppm and histone HCP below limit of detection (LOD). DNA was reduced to less than 1 ppb, and aggregates/free LC less than 0.1%. The overall IgG recovery was 87.2%. A simple and efficient purification platform with only one-column step was therefore established, providing a more promising alternative to the current prevailing protein A-based purification platforms. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | A AFFINITY-CHROMATOGRAPHY ; MIXED-MODE CHROMATOGRAPHY ; IMMUNOGLOBULIN-G ; BIOSEPARATION TECHNIQUE ; HUMAN-ALBUMIN ; FUTURE ; CAPTURE ; IGG ; MICROFILTRATION ; IDENTIFICATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000384852600013 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9082]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Agcy Sci Technol & Res, Bioproc Technol Inst, 20 Biopolis Way, Singapore 138668, Singapore |
| 推荐引用方式 GB/T 7714 | Chen, Quan,Latiff, Sarah Maria Abdul,Toh, Phyllicia,et al. A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography[J]. JOURNAL OF BIOTECHNOLOGY,2016,236:128-140. |
| APA | Chen, Quan.,Latiff, Sarah Maria Abdul.,Toh, Phyllicia.,Peng, Xinying.,Hoi, Aina.,...&Gagnon, Pete.(2016).A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography.JOURNAL OF BIOTECHNOLOGY,236,128-140. |
| MLA | Chen, Quan,et al."A simple and efficient purification platform for monoclonal antibody production based on chromatin-directed cell culture clarification integrated with precipitation and void-exclusion anion exchange chromatography".JOURNAL OF BIOTECHNOLOGY 236(2016):128-140. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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