Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism
文献类型：期刊论文
| 作者 | Chen, Quan1; Toh, Phyllicia2; Sun, Yue1; Latiff, Sarah Maria Abdul2; Hoi, Aina2; Xian, Mo1; Zhang, Haibo1; Nian, Rui1; Zhang, Wei2; Gagnon, Pete2 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 100期号:23页码:9933-9941 |
| 关键词 | Octanoic acid Agglomeration Histone IgG Light chain |
| 英文摘要 | Octanoic acid (OA) precipitation has long been used in protein purification. Recently, we reported a new cell culture clarification method for immunoglobulin G (IgG) purification, employing an advance elimination of chromatin heteroaggregates with a hybrid OA-solid phase system. This treatment reduced DNA more than 3 logs, histone below the detection limit (LOD), and non-histone host cell proteins (nh-HCP) by 90 % while conserving more than 90 % of the IgG monomer. In this study, we further investigated the conservation of IgG monomer and antibody light chain (LC) to the addition of OA/OA-solid phase complex, with or without histone and DNA in different combinations. The results showed that highly basic histone protein was the prime target in OA/OA-solid phase precipitation system for IgG purification, and the selective conservation of IgG monomer in this system was histone dependent. Our findings partially support the idea that OA works by sticking to electropositive hydrophobic domains on proteins, reducing their solubility, and causing them to agglomerate into large particles that precipitate from solution. Our findings also provide a new perspective for IgG purification and emphasize the necessity to re-examine the roles of various host contaminants in IgG purification. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | CHO-CELL LINES ; MONOCLONAL-ANTIBODY ; CAPRYLIC-ACID ; IMMUNOGLOBULIN-G ; LIGHT-CHAIN ; PROTEIN-A ; PURIFICATION ; CHROMATIN ; CHROMATOGRAPHY ; PERFORMANCE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387656500011 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9097]  |
| 专题 | 青岛生物能源与过程研究所_材料生物技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.ASTAR, Bioproc Technol Inst, Singapore, Singapore |
| 推荐引用方式 GB/T 7714 | Chen, Quan,Toh, Phyllicia,Sun, Yue,et al. Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(23):9933-9941. |
| APA | Chen, Quan.,Toh, Phyllicia.,Sun, Yue.,Latiff, Sarah Maria Abdul.,Hoi, Aina.,...&Gagnon, Pete.(2016).Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(23),9933-9941. |
| MLA | Chen, Quan,et al."Histone-dependent IgG conservation in octanoic acid precipitation and its mechanism".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.23(2016):9933-9941. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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