A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase
文献类型：期刊论文
| 作者 | Cheng Yu-rong1,3,4; Sun Zhi-jie1; Cui Gu-zhen1,3; Song Xiaojin1,3; Cui Qiu1,2,3 |
| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 93-94页码:182-190 |
| 关键词 | Aurantiochytrium sp. Heavy-ions mutagenesis Triclosan Isoniazid Coldstress |
| 英文摘要 | Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study investigated the growth and metabolism response of Aurantiochytrium sp. CGMCC 6208 to two inhibitors of enoyl-ACP reductase of Type II FAS pathway (isoniazid and triclosan), and proposed a method of screening high DHA yield Aurantiochytrium sp. strains with heavy ion mutagenesis and pre-selection by synergistic usage of cold stress (4 degrees C) and FAS inhibitors (triclosan and isoniazid). Results showed that (1) isoniazid and triclosan have positive effects on improving DHA level of cells; (2) mutants from irradiation dosage of 120 Gy yielded more DHA compared with cells from 40 Gy, 80 Gy treatment and wild type; (3) DHA contents of mutants pre-selected by inhibitors of enoyl-ACP reductase of Type II FAS pathway (isoniazid and triclosan)at 4 degrees C, were significantly higher than that of wild type; (4) compared to the wild type, the DHA productivity and yield of a mutant (T-99) obtained from Aurantiochytrium sp. CGMCC 6208 by the proposed method increased by 50% from 0.18 to 0.27 g/Lh and 30% from 21 to 27 g/L, respectively. In conclusion, this study developed a feasible method to screen Aurantiochytrium sp. with high DHA yield by a combination of heavy-ion mutagenesis and mutant-preselection by FAS inhibitors and cold stress. (C) 2016 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | FATTY-ACID BIOSYNTHESIS ; DOCOSAHEXAENOIC ACID ; SCHIZOCHYTRIUM SP ; ARABIDOPSIS-THALIANA ; GAMMA-IRRADIATION ; LOW-TEMPERATURE ; OIL PRODUCTION ; MUTATIONS ; NANNOCHLOROPSIS ; OPTIMIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000386317700021 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9069]  |
| 专题 | 青岛生物能源与过程研究所_代谢物组学团队 |
| 作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Shandong, Peoples R China 3.Qingdao Engn Lab Single Cell Oil, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng Yu-rong,Sun Zhi-jie,Cui Gu-zhen,et al. A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,93-94:182-190. |
| APA | Cheng Yu-rong,Sun Zhi-jie,Cui Gu-zhen,Song Xiaojin,&Cui Qiu.(2016).A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase.ENZYME AND MICROBIAL TECHNOLOGY,93-94,182-190. |
| MLA | Cheng Yu-rong,et al."A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase".ENZYME AND MICROBIAL TECHNOLOGY 93-94(2016):182-190. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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