Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome
文献类型：期刊论文
| 作者 | Teng, Lin1,2,3; Wang, Xian1,2,3; Wang, Xiaojun1,2,3 ; Gou, Honglei1,2; Ren, Lihui1,2; Wang, Tingting1,2; Wang, Yun1,2; Ji, Yuetong1,2; Huang, Wei E.4; Xu, Jian1,2
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016-10-19 |
| 卷号 | 6 |
| 英文摘要 | Rapid profiling of stress-response at single-cell resolution yet in a label-free, non-disruptive and mechanism-specific manner can lead to many new applications. We propose a single-cell-level biochemical fingerprinting approach named "ramanome", which is the collection of Single-cell Raman Spectra (SCRS) from a number of cells randomly selected from an isogenic population at a given time and condition, to rapidly and quantitatively detect and characterize stress responses of cellular population. SCRS of Escherichia coli cells are sensitive to both exposure time (eight time points) and dosage (six doses) of ethanol, with detection time as early as 5 min and discrimination rate of either factor over 80%. Moreover, the ramanomes upon six chemical compounds from three categories, including antibiotics of ampicillin and kanamycin, alcohols of ethanol and n-butanol and heavy metals of Cu2+ and Cr6+, were analyzed and 31 marker Raman bands were revealed which distinguish stress-responses via cytotoxicity mechanism and variation of inter-cellular heterogeneity. Furthermore, specificity, reproducibility and mechanistic basis of ramanome were validated by tracking stress-induced dynamics of metabolites and by contrasting between cells with and without genes that convey stress resistance. Thus ramanome enables rapid prediction and mechanism-based screening of cytotoxicity and stress-response programs at single-cell resolution. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | SINGLE YEAST-CELLS ; ESCHERICHIA-COLI ; MICROBIAL-CELLS ; ETHANOL STRESS ; SPECTROSCOPY ; IDENTIFICATION ; MICROSPECTROSCOPY ; COPPER ; MICROALGAE ; SIGNATURES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000385734500001 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9062]  |
| 专题 | 青岛生物能源与过程研究所_单细胞中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Oxford, Dept Engn, Parks Rd, Oxford OX1 3PJ, England |
| 推荐引用方式 GB/T 7714 | Teng, Lin,Wang, Xian,Wang, Xiaojun,et al. Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome[J]. SCIENTIFIC REPORTS,2016,6. |
| APA | Teng, Lin.,Wang, Xian.,Wang, Xiaojun.,Gou, Honglei.,Ren, Lihui.,...&Xu, Jian.(2016).Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome.SCIENTIFIC REPORTS,6. |
| MLA | Teng, Lin,et al."Label-free, rapid and quantitative phenotyping of stress response in E. coli via ramanome".SCIENTIFIC REPORTS 6(2016). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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