Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability
文献类型：期刊论文
| 作者 | Duan Yangkai1,2; Luo Quan1 ; Liang Feiyan1,2; Lu Xuefeng1
|
| 刊名 | JOURNAL OF OCEAN UNIVERSITY OF CHINA
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 15期号:5页码:890-896 |
| 关键词 | pre-treatment salt stress sps sucrose Synechococcus elongatus PCC 7942 |
| 英文摘要 | The unicellular cyanobacterium, Synechococcus elongatus PCC 7942 (Syn7942), synthesizes sucrose as the only compatible solute under salt stress. A series of engineered Syn7942 strains for sucrose production were constructed. The overexpression of the native sps (encoding a natively fused protein of sucrose phosphate synthase SPS and sucrose phosphate phosphatase SPP) in Syn7942 wild type caused a 93% improvement of sucrose productivity. The strain FL130 co-overexpressing sps and cscB (encoding a sucrose transporter) exhibited a 74% higher extracellular sucrose production than that overexpressing cscB only. Both results showed the significant improvement of sucrose productivity by the double functional protein SPS-SPP. Afterwards, FL130 was cultivated under a modified condition, and the cell-free culture medium containing 1.5 g L-1 sucrose was pre-treated with an acid hydrolysis technique. Cultivated with the neutralized hydrolysates as the starting media, two widely used microorganisms, Escherichia coli and Saccharomyces cerevisiae, showed a comparable growth with that in the control media supplemented with glucose. These results clearly demonstrated that the cell-free culture of sucrose-secreting cyanobacteria can be applied as starting media in microbial cultivation. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Oceanography |
| 研究领域[WOS] | Oceanography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000387574800018 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9096]  |
| 专题 | 青岛生物能源与过程研究所_微生物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan Yangkai,Luo Quan,Liang Feiyan,et al. Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(5):890-896. |
| APA | Duan Yangkai,Luo Quan,Liang Feiyan,&Lu Xuefeng.(2016).Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(5),890-896. |
| MLA | Duan Yangkai,et al."Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.5(2016):890-896. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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