Perfluorinated chemicals (PFCs) are new emerging organic pollutants. They have drawn increasing concerns because of their environmental persistence, bioaccumulation, biomagnification and biotoxicity. Application of biosolids to agricultural fields would cause serious PFCs pollution in surface soil and groundwater. Plant uptake and translocation of pollutants from soils is an important process which could introduce pollutants into food chain. Studies on this process for PFCs would shed light on the environmental behavior of PFCs, provide information for assessing the environmental and health risks of biosolids application, and help to get PFC-free crop products by means of blocking and genetic engineering technologies. However, information of the transport and transformation behavior of PFCs in the soil-plant system is quite limited so far. Accordingly, in this study, plant transport, translocation and metabolism of perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and N-ethyl perfluorooctane sulfonamido acetic acid (N-EtFOSAA) were studied. Following results were obtained. 1) Uptake, translocation and metabolism of PFOS, PFOA and N-EtFOSAA by seven types of plants, maize (Zea mays L.), soybean (Glycine max L. Merrill), radish (Raphnus sativus L.), green bean (glycine max Merr.), lettuce (Lactuca sativa L.), alfalfa(Medicago sativa L.) and ryegrass (Lolium multiflorum L.), from biosolid-amended soils were investigated. The PFCs tested had been found in the roots of all plants, with the maximum root concentration factors (RCFs) of 4.99, 10.18 and 1.37 for PFOS, PFOA and N-EtFOSAA, respectively, showing that PFCs could be transported from soil to plant. The RCF values of PFOA were higher than those of PFOS for green bean, lettuce, alfalfa and ryegrass, while the RCF values of PFOA were lower than those of PFOS for maize and soybean. No significant difference was found for RCF values between PFOS and PFOA for radish. Lettuce had the biggest RCF value for N-EtFOSAA (1.4), whereas green bean had the smallest RCF value for N-EtFOSAA (0.52). The RCF values of N-EtFOSAA were in the range from 0.6 to 1.0 for other five plants. PFOS and PFOA had been found in the stems and leaves of all plants tested, suggesting that PFOS and PFOA could be translocated. The translocation factors (TF) of PFOA were higher than PFOS for radish, green bean, lettuce, alfalfa and ryegrass. There is no difference between the TF values for maize and soybean. No N-EtFOSAA was found in stems and leaves of all plants, showing its weak translocation ability. The N-EtFOSAA degradation products, N-ethyl perfluorooctane sulfonamide (N-EtFOSA) and perfluorooctane sulfonamide (FOSA), had been found in all plant roots. N-EtFOSA was also found in all plant stems and leaves. But FOSA was only detected in the stem of ryegrass, which suggested that N-EtFOSAA could be degraded by plants studied, and the degradation ability of ryegrass was superior to the others. This work provided an evidence for plant uptake, translocation and degradation of PFCs from biosolid-amended soils. 2) A series of experiments were conducted in a hydroponic system to study the uptake and toxicity of PFOS and PFOA to maize (Zea mays L.). When PFOS or PFOA concentrations were higher than 1mg/L, maize dry weights of roots and shoots were inhibited significantly. When PFOS and PFOA concentrations were higher than 100mg/L, PFOA are more toxic than PFOS. Moreover, the ratio of shoot/root increased with PFOS and PFOS concentration increasing, suggesting the roots are more sensitive than shoots. Uptake of PFOS was notably inhibited by aquaporin inhibitors (Ag+ and glycerol) and anion channel blockers (4,4'- diisothiocyanostilbene-2,2'-disulfonic acid, DIDS; 5-Nitro-2- (3-phenylpropylamino) benzoic Acid, NPPB), indicating that aquaporin and anion channels were necessary in PFOS absorption by maize root. However, PFOS uptake into maize was insensitive to metabolic inhibitors (NaN3 and Na3VO4), suggesting that the transport of PFOS from solution to maize roots was an energy-independent process. In contrast, the uptake of PFOA was reduced by metabolic inhibitors, revealing its energy-dependent uptake nature. No absorption competition was found between PFOS and PFOA, which implied that the uptake of PFOS and PFOA followed different pathways. The concentrations of PFOS and PFOA in roots were decreased with temperature decreasing. No significant difference of uptake was found when the solution pH ranged from 5 to 8. To our knowledge, this is the first study on the absorption mechanism of PFOS and PFOA by plants.