Modification of the RpoS network with a synthetic small RNA
文献类型：期刊论文
| 作者 | Jin, Ye; Wu, Jianting; Li, Yannan; Cai, Zhiming; Huang, Jian-Dong |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2013 |
| 英文摘要 | Translation of the sigma factor RpoS is activated by DsrA, RprA and ArcA, three small non-coding sRNAs (sRNA) that expose the ribosome-binding site (RBS) by opening up an inhibitory loop. In the RpoS network, no sRNAs have been found to pair with the RBS, a most common sRNA target site in bacteria. Here, we generate Ribo-0, an artificial sRNA, which represses rpoS translation by pairing with the RBS. Ribo-0 bypasses the RNA chaperon Hfq but requires theRBS to be loosely blocked. Ribo-0 interacts with DsrA and reshapes the RpoS network. Specifically, in the intact RpoS network, DsrA activates rpoStranslation by freeing up the RBS. In the modified RpoS network where Ribo-0 is introduced, the DsrA-caused RBS exposure facilitates Ribo-0 binding, thereby strengthening Ribo-0 inhibition. In other words, Ribo-0 changes DsrA from an activator to an accomplice for repressing rpoS translation. This work presents anartificial mechanism of rpoS regulation, reveals mutual effects of native and synthetic players and demonstrates genetic context-dependency of their functions. |
| 收录类别 | SCI |
| 原文出处 | http://nar.oxfordjournals.org/content/41/17/8332 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5275]  |
| 专题 | 深圳先进技术研究院_南沙所 |
| 作者单位 | NUCLEIC ACIDS RESEARCH |
| 推荐引用方式 GB/T 7714 | Jin, Ye,Wu, Jianting,Li, Yannan,et al. Modification of the RpoS network with a synthetic small RNA[J]. NUCLEIC ACIDS RESEARCH,2013. |
| APA | Jin, Ye,Wu, Jianting,Li, Yannan,Cai, Zhiming,&Huang, Jian-Dong.(2013).Modification of the RpoS network with a synthetic small RNA.NUCLEIC ACIDS RESEARCH. |
| MLA | Jin, Ye,et al."Modification of the RpoS network with a synthetic small RNA".NUCLEIC ACIDS RESEARCH (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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