Immunomodulating polysaccharides from Lessertia frutescens leaves: Isolation, characterization and structure activity relationship
文献类型：期刊论文
| 作者 | Bingzhao Zhang; Wei Kee Leung; Yuanfeng Zou; Wilfred Mabusela; Quinton Johnson; Terje E. Michaelsen; Berit Smestad Paulsen |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2014 |
| 英文摘要 | Ethnopharmacological relevance: Sutherlandia frutescens (syn. Lessertia frutescens) is an indigenous plant in Southern Africa and has been extensively studied from the ethnobotanical point of view. Amongst the various traditional uses, several illnesses involving the immune system have been reported. Due to some of the therapeutic effects observed, in relation to the traditional uses reported by the "khoi san" and "nama" people on cancer related illnesses, the plant has been given the local name kankerbos (cancerbush). Recently the plant has also been used amongst HIV/AIDS patients to stimulate the immune system. Materials and methods: Leaves of Sutherlandia frutescens were extracted sequentially with ethanol, 50% ethanol/water, and water at 50 and 100 degrees C. The polysaccharides were extracted with water and fractionated by ion exchange chromatography and gel filtration to obtain enriched polysaccharide fractions. The bioactivities of the fractions were tested in the complement assay. Some of the fractions were treated with the enzyme pectinase, and the fragments thus produced were separated by gel filtration and their activities tested. Monosaccharide compositions and linkage analyses were determined for the relevant fractions. Results: The leaves of Sutherlandia frutescens contain polysaccharides of the pectin type. Fractions from both the water extracts of 50 and 100 degrees C were bioactive. Fractions chosen for further studies showed that the fragment with the highest M-W after the pectinase treatment had a substantially higher biological effect than the parent molecules. Based on a comparison of the different fractions it was concluded that galactose-rich regions were important for the bioactivity, these being of the AGII and AGI type, with the latter probably being more important than the former. Fragments rich in xylose also gave higher activity than those without it. Conclusions: Our theory that the polysaccharides present in the leaves of Sutherlandia frutescens could be of importance as immunomodulating agents was confirmed. It was also shown that certain types of polysaccharides had a higher effect in the complement system than others. Thus both the water extracts obtained at 50 and 100 degrees C contain interesting biologically active polysaccharides. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0378874114000634 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/6274]  |
| 专题 | 深圳先进技术研究院_南沙所 |
| 作者单位 | JOURNAL OF ETHNOPHARMACOLOGY |
| 推荐引用方式 GB/T 7714 | Bingzhao Zhang,Wei Kee Leung,Yuanfeng Zou,et al. Immunomodulating polysaccharides from Lessertia frutescens leaves: Isolation, characterization and structure activity relationship[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014. |
| APA | Bingzhao Zhang.,Wei Kee Leung.,Yuanfeng Zou.,Wilfred Mabusela.,Quinton Johnson.,...&Berit Smestad Paulsen.(2014).Immunomodulating polysaccharides from Lessertia frutescens leaves: Isolation, characterization and structure activity relationship.JOURNAL OF ETHNOPHARMACOLOGY. |
| MLA | Bingzhao Zhang,et al."Immunomodulating polysaccharides from Lessertia frutescens leaves: Isolation, characterization and structure activity relationship".JOURNAL OF ETHNOPHARMACOLOGY (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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