MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP
文献类型：期刊论文
| 作者 | Liang WC; Wang Y; Liang PP; Pan XQ; Fu WM; Yeung VS; Lu YF; Wan DC; Tsui SK; Tsang SY |
| 刊名 | JOURNAL OF CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2015 |
| 英文摘要 | In the past decade, miRNA emerges as a vital player in orchestrating gene regulation and maintaining cellular homeostasis. It is well documented that miRNA influences a variety of biological events, including embryogenesis, cell fate decision, and cellular differentiation. Adipogenesis is an organized process of cellular differentiation by which pre-adipocytes differentiate towards mature adipocytes. It has been shown that adipogenesis is tightly modulated by a number of transcription factors such as PPARγ, KLF4, and C/EBPα. However, the molecular mechanisms underlying the missing link between miRNA and adipogenesis-related transcription factors remain elusive. In this study, we unveiled that miR-25, a member of miR-106b-25 cluster, was remarkably downregulated during 3T3-L1 adipogenesis. Restored expression of miR-25 significantly impaired 3T3-L1 adipogenesis and downregulated the expression of serial adipogenesis-related genes. Further experiments presented that ectopic expression of miR-25 did not affect cell proliferation and cell cycle progression. Finally, KLF4 and C/EBPα, two key regulators of adipocyte differentiation, were experimentally identified as bona fide targets for miR-25. These data indicate that miR-25 is a novel negative regulator of adipocyte differentiation and it suppressed 3T3-L1 adipogenesis by targeting KLF4 and C/EBPα, which provides novel insights into the molecular mechanism of miRNA-mediated cellular differentiation. |
| 收录类别 | SCI |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pubmed/25923408 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7554]  |
| 专题 | 深圳先进技术研究院_南沙所 |
| 作者单位 | JOURNAL OF CELLULAR BIOCHEMISTRY |
| 推荐引用方式 GB/T 7714 | Liang WC,Wang Y,Liang PP,et al. MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2015. |
| APA | Liang WC.,Wang Y.,Liang PP.,Pan XQ.,Fu WM.,...&Waye M.(2015).MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP.JOURNAL OF CELLULAR BIOCHEMISTRY. |
| MLA | Liang WC,et al."MiR-25 suppresses 3T3-L1 adipogenesis by directly targeting KLF4 and C/EBP".JOURNAL OF CELLULAR BIOCHEMISTRY (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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