A centrifugal microfluidics platform for potential application on immobilization-free bead-based immunoassays
文献类型：会议论文
| 作者 | Chen, Qiu-lan; Cheung, Ka-lun; Kwan, Yiu-wa; Kong, Siu-kai; Ho, Ho-pui |
| 出版日期 | 2012 |
| 会议名称 | 2nd International Forum on Micro Manufacturing, IFMM 2012 |
| 会议地点 | S China Univ Techn, Guangzhou, PEOPLES R CHINA |
| 英文摘要 | In this work, we demonstrate a simple yet convenient centrifugal microfluidics, or "lab-on-a-disc (LOAD)" platform for bead-based immunoassays. The disc contains a network of passive valves for sample manipulation and a bead-aggregating barrier structure to instead of commonly used immobilization method. The narrow-channel structure not only provides a geometric barrier for retaining the beads, but also significantly increased fluidic resistance which results in lower flow rate and longer reaction time for each reagent in the reaction chamber. Centrifuge-forced beads densification in the small junction area and signal concentrating allows high signal sensing capability. The acceleration speed is optimized for largest signal-to-noise ratio. The device uses different rotation speed to trigger a cascade of flow events. The integrated platform contains all the necessary functional elements for conducting one-step parallel assays through a sequential protocol. For our test device, the total time required to complete an immunoassay experiment is typically under 6 minutes. Through this work we have also shown that the use of a geometric barrier for the aggregation of signaling beads is an effective approach for the enhancement of optical signals in common immunoassays. This flexible design is suitable for a broad range of bead-based immunoassays with low-cost and high-speed operation. |
| 收录类别 | EI |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4346]  |
| 专题 | 深圳先进技术研究院_南沙所 |
| 作者单位 | 2012 |
| 推荐引用方式 GB/T 7714 | Chen, Qiu-lan,Cheung, Ka-lun,Kwan, Yiu-wa,et al. A centrifugal microfluidics platform for potential application on immobilization-free bead-based immunoassays[C]. 见:2nd International Forum on Micro Manufacturing, IFMM 2012. S China Univ Techn, Guangzhou, PEOPLES R CHINA. |
入库方式： OAI收割
来源：深圳先进技术研究院
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