Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies
文献类型：期刊论文
| 作者 | Ma, Yunhan; Li, Jiayu; Wang, Ge; Ke, Qiong; Qiu, Sien; Gao, Liang; Wan, Haifeng; Zhou, Yang; Xiang, Andy Peng; Huang, Qunshan |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2016 |
| 英文摘要 | The efficiency of assisted reproductive technologies (ARTs) in nonhuman primates is low due to no screening criterions for selecting sperm, oocyte, and embryo as well as its surrogate mothers. Here we analyzed 15 pairs of pregnant and non-pregnant cynomolgus monkeys, each pair of which received embryos from one batch of fertilized oocytes, and found ratio of endometrial to myometrial thicknesses in abdominal ultrasonic transverse section of uterus is a reliable indicator for selection of recipients for embryo transfer. We performed 305 ovarian stimulations in 128 female cynomolgus monkeys and found that ovarian stimulation can be performed in a whole year and repeated up to six times in the same monkey without deteriorating fertilization potential of eggs until a poor response to stimulation happened. Fertilization can be efficiently achieved with both conventional and piezo-driven intracytoplasmic sperm injection procedures. In semen collection, semen quality is higher with the penile robe electrical stimulus method compared with the rectal probe method. Moreover, caesarean section is an effective strategy for increasing baby survival ratesof multiple pregnancies. These findings provide a practical guidance for the efficient use of ARTs, facilitating their use in genetic engineering of macaque monkeys for basic and translational neuroscience research. |
| 收录类别 | SCI |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865753/ |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/9786]  |
| 专题 | 深圳先进技术研究院_脑所 |
| 作者单位 | SCIENTIFIC REPORTS |
| 推荐引用方式 GB/T 7714 | Ma, Yunhan,Li, Jiayu,Wang, Ge,et al. Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies[J]. SCIENTIFIC REPORTS,2016. |
| APA | Ma, Yunhan.,Li, Jiayu.,Wang, Ge.,Ke, Qiong.,Qiu, Sien.,...&Yang, Shihua.(2016).Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies.SCIENTIFIC REPORTS. |
| MLA | Ma, Yunhan,et al."Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies".SCIENTIFIC REPORTS (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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