Cell cytotoxicity analysis of adherent and suspended cancer cells using an integrated microfluidic device
文献类型：会议论文
| 作者 | Song, Huixue; Peng, Shaoti; Chen, Tan; Zhang, Baoyue; Wang, Zhanhui |
| 出版日期 | 2011 |
| 会议名称 | International Conference on Biomedical Engineering and Informatics |
| 会议地点 | Shanghai |
| 英文摘要 | In this paper, a multilayer elastomeric microfluidic device with micro cell sieves was developed for an assay of drug induced cytotoxicity. We evaluated the cytotoxic effects of anti-cancer drugs tamoxifen (TAM) and camptothecin using MDA-MB-231 breast cancer cells and human chronic myeloid leukaemia K562 cells respectively. To validate cell morphology and viability on the microfluidic chip, fluorescent cell viability assay was carried out using three fluorescent dyes. The results showed that TAM and camptothecin both induced cytotoxicity of the two cell types in a concentration-dependent manner. Using this scalable platform, cell seeding and anti-cancer drugs exposure can be carried out within a single microfluidic device in a multiplexed format, enabling high-density parallel cytotoxicity screening while minimizing reagent consumption |
| 收录类别 | EI |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3496]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2011 |
| 推荐引用方式 GB/T 7714 | Song, Huixue,Peng, Shaoti,Chen, Tan,et al. Cell cytotoxicity analysis of adherent and suspended cancer cells using an integrated microfluidic device[C]. 见:International Conference on Biomedical Engineering and Informatics. Shanghai. |
入库方式： OAI收割
来源：深圳先进技术研究院
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