Evaluation of normalization methods for RNA-Seq gene expression estimation
文献类型：会议论文
| 作者 | Wu po-yen; Phan John H.; Zhou fengfeng; Wang may D. |
| 出版日期 | 2011 |
| 会议名称 | 2011 IEEE International Conference onBioinformatics and Biomedicine Workshops, BIBMW 2011 |
| 会议地点 | Atlanta, GA, USA |
| 英文摘要 | Statistical inferences on RNA-Seq data, e.g., detecting differential gene expression, are meaningful only after proper normalization. However, there is no consensusfor choosing a normalization procedure from among the many existing procedures. We evaluated several RNA-Seq normalization procedures by (1) correlatingestimated RNA-Seq expression values to those of microarrays, (2) examining the concordance of stable and differential gene detection between the platforms, and (3) applying the procedures to simulated RNA-Seq data. Results suggested that RNA-Seq normalization procedures have little effect on both inter-platform geneexpression correlation as well as inter-platform concordance of genes detected as stably or differentially expressed. However, the results of simulated analysis suggested that some normalization procedures are more robust to changes in distribution of differentially expressed genes. These results may provide guidance forselecting RNA-Seq normalization procedures. © 2011 IEEE.(24 refs) |
| 收录类别 | EI |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3525]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2011 |
| 推荐引用方式 GB/T 7714 | Wu po-yen,Phan John H.,Zhou fengfeng,et al. Evaluation of normalization methods for RNA-Seq gene expression estimation[C]. 见:2011 IEEE International Conference onBioinformatics and Biomedicine Workshops, BIBMW 2011. Atlanta, GA, USA. |
入库方式： OAI收割
来源：深圳先进技术研究院
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