Two-photon excited fluorescence emission from hemoglobin
文献类型：会议论文
| 作者 | Sun Qiqi; Zeng Yan; Zhang Wei; Zheng Wei; Luo Yi; Qu Jianan Y. |
| 出版日期 | 2015 |
| 会议名称 | Conference on Multiphoton Microscopy in the Biomedical Sciences XV 2015 |
| 会议地点 | 9329 |
| 英文摘要 | Hemoglobin, one of the most important proteins in blood, is responsible for oxygen transportation in almost all vertebrates. Recently, we discovered two-photon excited hemoglobin fluorescence and achieved label-free microvascular imaging based on the hemoglobin fluorescence. However, the mechanism of its fluorescence emission still remains unknown. In this work, we studied the two-photon excited fluorescence properties of the hemoglobin subunits, heme/hemin (iron (II)/(III) protoporphyrin IX) and globin. We first studied the properties of heme and the similar spectral and temporal characteristics of heme and hemoglobin fluorescence provide strong evidence that heme is the fluorophore in hemoglobin. Then we studied the fluorescence properties of hemin, globin and methemoglobin, and found that the hemin may have the main effect on the methemoglobin fluorescence and that globin has tryptophan fluorescence like other proteins. Finally, since heme is a centrosymmetric molecule, that the Soret band fluorescence of heme and hemoglobin was not observed in the single photon process in the previous study may be due to the parity selection rule. The discovery of heme two-photon excited fluorescence may open a new window for heme biology research, since heme as a cofactor of hemoprotein has many functions, including chemical catalysis, electron transfer and diatomic gases transportation. |
| 收录类别 | ISTP |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7212]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2015 |
| 推荐引用方式 GB/T 7714 | Sun Qiqi,Zeng Yan,Zhang Wei,et al. Two-photon excited fluorescence emission from hemoglobin[C]. 见:Conference on Multiphoton Microscopy in the Biomedical Sciences XV 2015. 9329. |
入库方式： OAI收割
来源：深圳先进技术研究院
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