Study of Cucurbitacin E as a novel anti-rheumatic agent via inhibiting NF-κB pathway
文献类型：会议论文
| 作者 | Wenxiang Chenga; Qingyun Jia; , Ye Yue; Yingping Hua; Zhanwang Xu; Peng Zhang |
| 出版日期 | 2015 |
| 会议名称 | 2015 SICOT |
| 会议地点 | 广州 |
| 英文摘要 | Objective: Increasing studies indicated that Cucurbitacin E (CuE), a derivative isolated from Cucurbitaceae plants may exert anti-inflammatory effect. In this study we explored the effect of CuE on TNF-α-induced inflammatory cytokines production in human synoviocyte MH7A cells, and further explored the detailed molecular mechanism involved in its anti-inflammatory effect. Materials and methods: MH7A cells were stimulated with TNF-α in the presence or absence of CuE, The expression of pro-inflammatory cytokines was determined by quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA). Signal-transduction protein expression was determined by Western blot. Nuclear translocation of NF-κB p65 was determined by a confocal laserscanningfluorescence microscopy. Results: CuE suppressed TNF-α-induced interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-8 (IL-8) mRNA and protein expression in MH7A cells in a dose-dependent manner with no apparent cytotoxicity. CuE also suppresses TNF-α-induced phosphorylation of NF-κBp65, IKKα/β, and IκBαin a dose-and time-dependent manner as well as NF-κBp65 nuclear translocation. |
| 收录类别 | 其他 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7310]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | 2015 |
| 推荐引用方式 GB/T 7714 | Wenxiang Chenga,Qingyun Jia,, Ye Yue,et al. Study of Cucurbitacin E as a novel anti-rheumatic agent via inhibiting NF-κB pathway[C]. 见:2015 SICOT. 广州. |
入库方式： OAI收割
来源：深圳先进技术研究院
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