The role of surface charge in cationic liposome-induced antigen-presenting cell (APC) activation and viability
文献类型：期刊论文
| 作者 | Yifan Ma; Xiaofang Xie; Yan Zhuang; Dongmei Zhou; and Lintao Cai |
| 刊名 | The Journal of Immunology
 |
| 出版日期 | 2010 |
| 英文摘要 | Cationic liposomes, an effective carrier for drugs and gene delivery, have recently been reported as a potential vaccine adjuvant. Since the immuno-stimulatory effect of cationic liposomes may be related to their surface charge, we hereby investigated the effect of liposome charge on antigen-presenting cell (APC) activation and viability. The charge of cationic liposomes was adjusted by incorporating into DOTAP (a cationic liposome) variable amounts of DOPC (a neutral lipid). DOPC by itself did not affect monocyte activation or DC maturation. The DOTAP/DOPC liposomes with molar ratios of 4:1, 3:2 or 2:3 not only elevated CD86 on monocytes and induced the production TNF-alpha, but also significantly augmented the expression of CD83 and CD86 on both human and mouse dendritic cells (DCs). However, further increasing the proportion of DOPC significantly attenuated monocyte activation and DC maturation induced by DOTAP/DOPC liposomes. Interestingly, although DOTAP had a higher zeta potential than the DOTAP/DOPC liposome (4:1), it was less potent in inducing monocyte activation and TNF-alpha production. Moreover, the treatment of monocytes with DOTAP for 24 hr caused about 40% apoptosis, while the presence of DOPC significantly increased monocyte viability. Hence, an appropriate surface charge is crucial for enhancing the adjuvant effect of cationic liposomes and reducing their potential cytotoxicity. |
| 收录类别 | SCI |
| 原文出处 | http://www.jimmunol.org/cgi/content/meeting_abstract/184/1_MeetingAbstracts/98.21?sid=978d0113-bd02-4a03-b2cb-53a250cf84ca |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/2916]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | The Journal of Immunology |
| 推荐引用方式 GB/T 7714 | Yifan Ma,Xiaofang Xie,Yan Zhuang,et al. The role of surface charge in cationic liposome-induced antigen-presenting cell (APC) activation and viability[J]. The Journal of Immunology,2010. |
| APA | Yifan Ma,Xiaofang Xie,Yan Zhuang,Dongmei Zhou,&and Lintao Cai.(2010).The role of surface charge in cationic liposome-induced antigen-presenting cell (APC) activation and viability.The Journal of Immunology. |
| MLA | Yifan Ma,et al."The role of surface charge in cationic liposome-induced antigen-presenting cell (APC) activation and viability".The Journal of Immunology (2010). |
入库方式： OAI收割
来源：深圳先进技术研究院
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