Chemiluminescence response of Murine Macrophages on Multilayer Microfluidic Chips
文献类型：期刊论文
| 作者 | Linfen Yu; Qun Li; Hongwei Gai; Zhanhui Wang |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2012 |
| 英文摘要 | We have demonstrated an integrated platform for microfluidics and chemiluminescence (CL) detection that is capable of parallel cell culture, convenient liquid manipulation, and sensitive chemiluminescent detection. Luminol-dependent CL responses induced by three different stimuli, phytohemagglutinin (PHA), concanavalin A (ConA), and lipopolysaccharides (LPS), which can evoke a CL response in macrophages, were evaluated on this microfluidic chip. We studied the dose-dependent effect of these three stimuli on CL response in murine macrophages. PHA produced the highest CL response compared to LPS and ConA. The CL intensity produced by PHA was 6.85 and four times higher than that by LPS and ConA, respectively, at the low concentration of 100 mu g/ml. We have found microfluidic based CL to be a very useful screening tool, which is less laborious and more sensitive. This microfluidic system is disposable and capable of rapid device prototyping; it may prove to be very useful in clinical and pharmaceutical applications. |
| 收录类别 | SCI |
| 原文出处 | http://download-v2.springer.com/static/pdf/235/art%253A10.1007%252Fs12010-011-9467-9.pdf?token2=exp=1432478037~acl=%2Fstatic%2Fpdf%2F235%2Fart%25253A10.1007%25252Fs12010-011-9467-9.pdf*~hmac=6028506fdb1c8455671fca6db63b0e9e65497c44c0f534f7761109469fac0713 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3927]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY |
| 推荐引用方式 GB/T 7714 | Linfen Yu,Qun Li,Hongwei Gai,et al. Chemiluminescence response of Murine Macrophages on Multilayer Microfluidic Chips[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012. |
| APA | Linfen Yu,Qun Li,Hongwei Gai,&Zhanhui Wang.(2012).Chemiluminescence response of Murine Macrophages on Multilayer Microfluidic Chips.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY. |
| MLA | Linfen Yu,et al."Chemiluminescence response of Murine Macrophages on Multilayer Microfluidic Chips".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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