An automated microfluidic device for assessment of mannalian cell genetic stability
文献类型：期刊论文
| 作者 | Yan Chen; Baoyue Zhang; Hongtao Feng; Weiliang Shu; Gina Y. Chen; Jiang F.Zhong |
| 刊名 | LAB ON A CHIP
 |
| 出版日期 | 2012 |
| 英文摘要 | Single-cell transcriptome contains reliable gene regulatory relationships because gene-gene interactions only happen within a mammalian cell. While the study of gene-gene interactions enables us to understand the molecular mechanism of cellular events and evaluate molecular characteristics of a mammalian cell population, its complexity requires an analysis of a large number of single-cells at various stages. However, many existing microfluidic platforms cannot process single-cells effectively for routine molecular analysis. To address these challenges, we develop an integrated system with individual controller for effective single-cell transcriptome analysis. In this paper, we report an integrated microfluidic approach to rapidly measure gene expression in individual cells for genetic stability assessment of a cell population. Inside this integrated microfluidic device, the cells are individually manipulated and isolated in an array using micro sieve structures, then transferred into different nanoliter reaction chambers for parallel processing of single-cell transcriptome analysis. This device enables us to manipulate individual single-cells into nanoliter reactor with high recovery rate. We have performed gene expression analysis for a large number of HeLa cells and 293T cells expanded from a single-cell. Our data shows that even the house-keeping genes are expressed at heterogeneous levels within a clone of cells. The heterogeneity of actin expression reflects the genetic stability, and the expression distribution is different between cancer cells (HeLa) and immortalized 293T cells. The result demonstrates that this platform has the potential for assessment of genetic stability in cancer diagnosis. |
| 收录类别 | SCI |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447114/pdf/nihms-394651.pdf |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3929]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | LAB ON A CHIP |
| 推荐引用方式 GB/T 7714 | Yan Chen,Baoyue Zhang,Hongtao Feng,et al. An automated microfluidic device for assessment of mannalian cell genetic stability[J]. LAB ON A CHIP,2012. |
| APA | Yan Chen,Baoyue Zhang,Hongtao Feng,Weiliang Shu,Gina Y. Chen,&Jiang F.Zhong.(2012).An automated microfluidic device for assessment of mannalian cell genetic stability.LAB ON A CHIP. |
| MLA | Yan Chen,et al."An automated microfluidic device for assessment of mannalian cell genetic stability".LAB ON A CHIP (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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