A Microfluidic Platform for Multi-antigen immunofluorescence assays
文献类型：期刊论文
| 作者 | Baoyue Zhang; Huixue Song; Tan Chen; Zhanhui Wang |
| 刊名 | MECHANICAL ENGINEERING AND MATERIALS SCIENCE
 |
| 出版日期 | 2012 |
| 英文摘要 | Traditional cell-based assays such as cell immunoassay that utilizes plastic (chamber slides, dishes, microtiter plates), Magnetic bead, enzyme-linked immunsorbent assays (ELISA) [1], FACS cell sorting is labor intensive, time consuming, and requires a large numbers of cells or reagents. In this report, a microfluidic device integrated with cell culture, washing, fixation, and antigen-antibody reaction is presented for high-throughput immunoassay. Using this microfluidic device, each assay can be performed on a small number of cells and nanolitre or picolitre of reagents, this is particularly beneficial for rare or expensive cell types such as stem cells, or flow sorted cell populations. The capability of the microfluidic device was demonstrated for seeding human umbilical cord blood mesenchymal stem cells (UC-MSCs) in chambers and detecting the expression of surface markers (CD34, CD44, CD45, CD73, CD15, HLA-DR) by immunofluorescence assay. |
| 收录类别 | EI |
| 原文出处 | http://www.scientific.net/AMM.108.200 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3931]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | MECHANICAL ENGINEERING AND MATERIALS SCIENCE |
| 推荐引用方式 GB/T 7714 | Baoyue Zhang,Huixue Song,Tan Chen,et al. A Microfluidic Platform for Multi-antigen immunofluorescence assays[J]. MECHANICAL ENGINEERING AND MATERIALS SCIENCE,2012. |
| APA | Baoyue Zhang,Huixue Song,Tan Chen,&Zhanhui Wang.(2012).A Microfluidic Platform for Multi-antigen immunofluorescence assays.MECHANICAL ENGINEERING AND MATERIALS SCIENCE. |
| MLA | Baoyue Zhang,et al."A Microfluidic Platform for Multi-antigen immunofluorescence assays".MECHANICAL ENGINEERING AND MATERIALS SCIENCE (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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