Toll-like receptor 3 agonist complexed with cationic liposome augments vaccine-elicited antitumor immunity by enhancing TLR3-IRF3 signaling and type I interferons in dendritic cells
文献类型：期刊论文
| 作者 | Ce Wang; Yan Zhuang; Yijuan Zhang; Zichao Luo; Ningning Gao; Ping Li; Hong Pan; Lintao Cai; Yifan Ma |
| 刊名 | Vaccine
 |
| 出版日期 | 2012 |
| 英文摘要 | Cancer vaccine-based immunotherapy is emerging as a novel therapeutic strategy for cancer treatment. However, its antitumor effect remains unsatisfied due to the poor immunogenicity of tumor antigens (Ags). Although polyriboinosinic: polyribocytidylic acid (PIC), a TLR3 agonist, has been reported as a promising adjuvant for cancer vaccines, its immunopotency may be limited by insufficient cellular penetration. In the present study, we incorporated PLC into DOTAP cationic liposome to generate PIC-DOTAP Liposome Complex (PDLC) nanoparticles. The results showed that PDLC was more potent than DOTAP or PIC to enhance vaccine-induced tumor-specific cytotoxic T lymphocyte (CTL) response and IFN-gamma production. Moreover, two doses of PDLC vaccines remarkably suppressed tumor growth in mice, which involved the participance of CD8(+) T cells and depended on the presence of Ag. The superior antitumor effect of PDLC vaccines could be attributable to enhanced maturation of mouse bone-marrow dendritic cells (BMDCs) and increased production of type I IFNs. More importantly. PDLC strengthened the TLR3 signaling in BMDCs by enhancing the interaction of PLC with TLR3 and augmenting downstream IRF-3 phosphorylation, as well as elevating IRF-3/IRF-7 mRNA transcription. Taking together, the complex of PLC and DOTAP liposomes enhanced PIC uptake and consequential TLR3 signaling in BMDCs, which in turn promoted DC maturation and type I IFN production, thereby augmenting the antitumor effect of cancer vaccines. |
| 收录类别 | SCI |
| 原文出处 | http://ac.els-cdn.com/S0264410X12007311/1-s2.0-S0264410X12007311-main.pdf?_tid=445bd100-079b-11e5-8bf8-00000aab0f01&acdnat=1433080095_8dd8f9f3b95a972970f6ba93580b0160 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3933]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | Vaccine |
| 推荐引用方式 GB/T 7714 | Ce Wang,Yan Zhuang,Yijuan Zhang,et al. Toll-like receptor 3 agonist complexed with cationic liposome augments vaccine-elicited antitumor immunity by enhancing TLR3-IRF3 signaling and type I interferons in dendritic cells[J]. Vaccine,2012. |
| APA | Ce Wang.,Yan Zhuang.,Yijuan Zhang.,Zichao Luo.,Ningning Gao.,...&Yifan Ma.(2012).Toll-like receptor 3 agonist complexed with cationic liposome augments vaccine-elicited antitumor immunity by enhancing TLR3-IRF3 signaling and type I interferons in dendritic cells.Vaccine. |
| MLA | Ce Wang,et al."Toll-like receptor 3 agonist complexed with cationic liposome augments vaccine-elicited antitumor immunity by enhancing TLR3-IRF3 signaling and type I interferons in dendritic cells".Vaccine (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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