PEGylated cationic liposomes robustly augment vaccine-induced immune responses: Role of lymphatic trafficking and biodistribution
文献类型：期刊论文
| 作者 | Yan Zhuang; Yifan Ma; Ce Wang; Luo Hai; Chao Yan; Yijuan Zhang; Fengzhi Liu; Lintao Cai |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2012 |
| 英文摘要 | Lymph nodes (LNs) are peripheral lymphoid organs essential for vaccine-induced immune responses. Although cationic liposomes have been documented as a novel adjuvant and vaccine delivery system, whether enhancing LN targeting would improve the efficiency of cationic liposome-formulated vaccines has not been elucidated yet. In the present study we investigated the effect of PEGylation on LN targeting and the immunogenicity of cationic liposome-formulated vaccines. DOTAP cationic liposomes were incorporated with 1 or 5 mol% of DSPE-PEG2000 and labeled with near infrared fluorescent dyes. The lymphatic trafficking and biodistribution of different liposomes after subcutaneous (s.c.) injection were recorded using an in-vivo imaging system. The results showed that incorporation of 1 mol% DSPE-PEG2000 not only accelerated the drainage of DOTAP liposomes into draining LNs, but also prolonged their LN retention and enhanced liposome uptake by resident antigen-presenting cells. On the other hand, although incorporating 5 mol% of DSPE-PEG2000 into DOTAP liposomes enhanced their LN retention and uptake to a lesser extent, it prolonged blood circulation of DOTAP liposomes and increased their splenic accumulation. In addition, PEGylated DOTAP liposomes augmented primary and secondary anti-OVA antibody responses more potently than nonPEGylated DOTAP liposomes did. Hence, incorporating a small amount of DSPE-PEG2000 into DOTAP liposomes not only increased the passive LN targeting of DOTAP-formulated vaccines but also modulated their biodistribution in vivo, which consequently improved the efficiency of cationic liposome-formulated vaccines. |
| 收录类别 | SCI |
| 原文出处 | http://ac.els-cdn.com/S0168365911011436/1-s2.0-S0168365911011436-main.pdf?_tid=2a320d34-079c-11e5-b22b-00000aab0f02&acdnat=1433080480_f4c32322e621f5d97b7b11922f312e23 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3934]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | JOURNAL OF CONTROLLED RELEASE |
| 推荐引用方式 GB/T 7714 | Yan Zhuang,Yifan Ma,Ce Wang,et al. PEGylated cationic liposomes robustly augment vaccine-induced immune responses: Role of lymphatic trafficking and biodistribution[J]. JOURNAL OF CONTROLLED RELEASE,2012. |
| APA | Yan Zhuang.,Yifan Ma.,Ce Wang.,Luo Hai.,Chao Yan.,...&Lintao Cai.(2012).PEGylated cationic liposomes robustly augment vaccine-induced immune responses: Role of lymphatic trafficking and biodistribution.JOURNAL OF CONTROLLED RELEASE. |
| MLA | Yan Zhuang,et al."PEGylated cationic liposomes robustly augment vaccine-induced immune responses: Role of lymphatic trafficking and biodistribution".JOURNAL OF CONTROLLED RELEASE (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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