Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors
文献类型：期刊论文
| 作者 | Yafeng Liu; Yuan Zhao; Xiaohua Lv; Yiding Li; Xiaohui Zhang; Jie Zhang; Liping Wang; Shaoqun Zeng |
| 刊名 | REVIEW OF SCIENTIFIC INSTRUMENTS
 |
| 出版日期 | 2012 |
| 英文摘要 | Recently developed optogenetics provides a fast, non-invasive, and efficient method for cell activation. However, it is difficult for the optical stimulators used for optogenetics to realize selective multisite fast activation. In this paper, we developed a random-access photostimulator based on a pair of perpendicularly oriented acousto-optic deflectors. Precise laser targeting in the x-y plane was verified, and the lateral spatial resolution of laser intensity after the objective was measured as similar to 1.38 mu m. Photostimulaton of ChETA-expressing astrocytes induced reliable inward currents only if the laser beam was directed onto the targeted cell. In the ChR2-expresing neuron, multiple locations along two dendrites were stimulated, and spatiotemporal integration was observed in the soma with fast multi-site activation. These results demonstrated that this random-access photostimulator would be a powerful tool for selective multi-site fast activation. The compact and modular design of this photostimulator makes it easily integrated with different commercial microscopes, and thus widely popularized in many laboratories. (C) 2012 American Institute of Physics. |
| 收录类别 | SCI |
| 原文出处 | http://scitation.aip.org/docserver/fulltext/aip/journal/rsi/83/2/1.3689954.pdf?expires=1433163722&id=id&accname=2113437&checksum=6044E101EEBA0CC2E64AB000ECD1D779 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3966]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | REVIEW OF SCIENTIFIC INSTRUMENTS |
| 推荐引用方式 GB/T 7714 | Yafeng Liu,Yuan Zhao,Xiaohua Lv,et al. Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2012. |
| APA | Yafeng Liu.,Yuan Zhao.,Xiaohua Lv.,Yiding Li.,Xiaohui Zhang.,...&Shaoqun Zeng.(2012).Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors.REVIEW OF SCIENTIFIC INSTRUMENTS. |
| MLA | Yafeng Liu,et al."Instrumentation of a compact random-access photostimulator based on acousto-optic deflectors".REVIEW OF SCIENTIFIC INSTRUMENTS (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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