cAMP/Protein Kinase A Activates Cystic Fibrosis Transmembrane Conductance Regulator for ATP Release from Rat Skeletal Muscle during Low pH or Contractions
文献类型：期刊论文
| 作者 | Jie Tu; Lin Lu; Weisong Cai; Ballard, Heather J |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012 |
| 英文摘要 | We have shown that cystic fibrosis transmembrane conductance regulator (CFTR) is involved in ATP release from skeletal muscle at low pH. These experiments investigate the signal transduction mechanism linking pH depression to CFTR activation and ATP release, and evaluate whether CFTR is involved in ATP release from contracting muscle. Lactic acid treatment elevated interstitial ATP of buffer-perfused muscle and extracellular ATP of L6 myocytes: this ATP release was abolished by the non-specific CFTR inhibitor, glibenclamide, or the specific CFTR inhibitor, CFTRinh-172, suggesting that CFTR was involved, and by inhibition of lactic acid entry to cells, indicating that intracellular pH depression was required. Muscle contractions significantly elevated interstitial ATP, but CFTRinh-172 abolished the increase. The cAMP/PKA pathway was involved in the signal transduction pathway for CFTR-regulated ATP release from muscle: forskolin increased CFTR phosphorylation and stimulated ATP release from muscle or myocytes; lactic acid increased intracellular cAMP, pCREB and PKA activity, whereas IBMX enhanced ATP release from myocytes. Inhibition of PKA with KT5720 abolished lactic-acid-or contraction-induced ATP release from muscle. Inhibition of either the Na+/H+-exchanger (NHE) with amiloride or the Na+/Ca2+-exchanger (NCX) with SN6 or KB-R7943 abolished lactic-acid-or contraction-induced release of ATP from muscle, suggesting that these exchange proteins may be involved in the activation of CFTR. Our data suggest that CFTR-regulated release contributes to ATP release from contracting muscle in vivo, and that cAMP and PKA are involved in the activation of CFTR during muscle contractions or acidosis; NHE and NCX may be involved in the signal transduction pathway. |
| 收录类别 | SCI |
| 原文出处 | http://apps.webofknowledge.com/OutboundService.do?&SID=Y1dItPWQXS8couN4PRw&publisher_id=DOISource&toPID=DOISource&URL=http%3A%2F%2Fgateway.webofknowledge.com%2Fgateway%2FGateway.cgi%3FGWVersion%3D2%26SrcAuth%3DDOISource%26SrcApp%3DWOS%26KeyAID%3D10.1371%252Fjournal.pone.0050157%26DestApp%3DDOI%26SrcAppSID%3DY1dItPWQXS8couN4PRw%26SrcJTitle%3DPLOS%2520ONE&product=WOS&action=go&mode=transferToPublisher&highlighted_tab=WOS&link_type=FULL_TEXT&PMID=&qid=59&doc=1&excludeEventConfig=ExcludeIfFromFullRecPage |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3972]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | PLOS ONE |
| 推荐引用方式 GB/T 7714 | Jie Tu,Lin Lu,Weisong Cai,et al. cAMP/Protein Kinase A Activates Cystic Fibrosis Transmembrane Conductance Regulator for ATP Release from Rat Skeletal Muscle during Low pH or Contractions[J]. PLOS ONE,2012. |
| APA | Jie Tu,Lin Lu,Weisong Cai,&Ballard, Heather J.(2012).cAMP/Protein Kinase A Activates Cystic Fibrosis Transmembrane Conductance Regulator for ATP Release from Rat Skeletal Muscle during Low pH or Contractions.PLOS ONE. |
| MLA | Jie Tu,et al."cAMP/Protein Kinase A Activates Cystic Fibrosis Transmembrane Conductance Regulator for ATP Release from Rat Skeletal Muscle during Low pH or Contractions".PLOS ONE (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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