A delivery system targeting bone formation surfaces to facilitate RNAi-based anabolic therapy
文献类型：期刊论文
| 作者 | Ge Zhang; Baosheng Guo; Heng Wu; Tao Tang; Bao-Ting Zhang; Lizhen Zheng; Yixin He; Zhijun Yang; Xiaohua Pan; Heelum Chow |
| 刊名 | NATURE MEDICINE
 |
| 出版日期 | 2012 |
| 英文摘要 | Metabolic skeletal disorders associated with impaired bone formation are a major clinical challenge. One approach to treat these defects is to silence bone-formation–inhibitory genes by small interference RNAs (siRNAs) in osteogenic-lineage cells that occupy the niche surrounding the bone-formation surfaces. We developed a targeting system involving dioleoyl trimethylammonium propane (DOTAP)-based cationic liposomes attached to six repetitive sequences of aspartate, serine, serine ((AspSerSer)6) for delivering siRNAs specifically to bone-formation surfaces. Using this system, we encapsulated an osteogenic siRNA that targets casein kinase-2 interacting protein-1 (encoded by Plekho1, also known as Plekho1). In vivo systemic delivery of Plekho1 siRNA in rats using our system resulted in the selective enrichment of the siRNAs in osteogenic cells and the subsequent depletion of Plekho1. A bioimaging analysis further showed that this approach markedly promoted bone formation, enhanced the bone micro-architecture and increased the bone mass in both healthy and osteoporotic rats. These results indicate (AspSerSer)6-liposome as a promising targeted delivery system for RNA interference–based bone anabolic therapy. |
| 收录类别 | SCI |
| 原文出处 | http://www.nature.com/nm/journal/v18/n2/abs/nm.2617.html |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4032]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | NATURE MEDICINE |
| 推荐引用方式 GB/T 7714 | Ge Zhang,Baosheng Guo,Heng Wu,et al. A delivery system targeting bone formation surfaces to facilitate RNAi-based anabolic therapy[J]. NATURE MEDICINE,2012. |
| APA | Ge Zhang.,Baosheng Guo.,Heng Wu.,Tao Tang.,Bao-Ting Zhang.,...&Ling Qin.(2012).A delivery system targeting bone formation surfaces to facilitate RNAi-based anabolic therapy.NATURE MEDICINE. |
| MLA | Ge Zhang,et al."A delivery system targeting bone formation surfaces to facilitate RNAi-based anabolic therapy".NATURE MEDICINE (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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