FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-beta-CyD
文献类型：期刊论文
| 作者 | Yiping Hu; Guping Tang; Jun Liu; Wenxiang Cheng; Ye Yue; Jinchao Li; Peng Zhang |
| 刊名 | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY
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| 出版日期 | 2012 |
| 英文摘要 | A novel vector with high gene delivery efficiency and special cell-targeting ability was developed using a good strategy that utilized low-molecular-weight polyethylenimine (PEI; molecular weight: 600 KDa [PEI600]) crosslinked to β-cyclodextrin (β-CyD) via a facile synthetic route. Fibroblast growth factor receptors (FGFRs) are highly expressed in a variety of human cancer cells and are potential targets for cancer therapy. In this paper, CY11 peptides, which have been proven to combine especially with FGFRs on cell membranes were coupled to PEI-β-CyD using N-succinimidyl-3-(2-pyridyldithio) propionate as a linker. The ratios of PEI600, β-CyD, and peptide were calculated based on proton integral values obtained from the 1H-NMR spectra of the resulting products. Electron microscope observations showed that CY11-PEI-β-CyD can efficiently condense plasmid DNA (pDNA) into nanoparticles of about 200 nm, and MTT assays suggested the decreased toxicity of the polymer. Experiments on gene delivery efficiency in vitro showed that CY11-PEI-β-CyD/pDNA polyplexes had significantly greater transgene activities than PEI-β-CyD/pDNA in the COS-7 and HepG2 cells, which positively expressed FGFR, whereas no such effect was observed in the PC-3 cells, which negatively expressed FGFR. Our current research indicated that the synthesized nonviral vector shows improved gene delivery efficiency and targeting specificity in FGFR-positive cells. |
| 收录类别 | SCI |
| 原文出处 | http://www.hindawi.com/journals/bmri/2012/989235/ |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4034]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY |
| 推荐引用方式 GB/T 7714 | Yiping Hu,Guping Tang,Jun Liu,et al. FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-beta-CyD[J]. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY,2012. |
| APA | Yiping Hu.,Guping Tang.,Jun Liu.,Wenxiang Cheng.,Ye Yue.,...&Peng Zhang.(2012).FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-beta-CyD.JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY. |
| MLA | Yiping Hu,et al."FGF Receptor-Mediated Gene Delivery Using Ligands Coupled to PEI-beta-CyD".JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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