Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion
文献类型：期刊论文
| 作者 | Lin Gao; Lisi Xie; Xiaojing Long; Zhiyong Wang; Cheng-Yi He; Zhi-Ying Chen; Lei Zhang; Xiang Nan; Hulong Lei; Xin Liu |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2013 |
| 英文摘要 | Gene therapy is a very promising technology for treatment of liver diseases. Minicircle DNA (MCDNA) is a versatile gene vector, which possesses excellent features in bio-safety and duration of transgene expression. However, its application has been hampered by the lack of an efficient gene delivery system. In this study, we developed a magnetic resonance imaging (MRI) visible nanoparticle to monitor MCDNA gene delivery and explore the potential of gene therapy in vivo. The nanoparticle was constructed via self-assembly of stearic acid modified low molecular polyethyleneimine (stPEI) and superparamagnetic iron oxide (SPIO) nanocrystals. The multiple SPIO nanocrystals with a controlled clustering structure in the nanoparticles were designed to achieve high MRI sensitivity. Furthermore, the MCDNA was combined with the nanoparticles via electronic interaction. Through intrabiliary infusion, the stPEI-SPIO/MCDNA nanoparticles were efficiently delivered to liver that was visualized by MRI in vivo and confirmed by histology. Moreover, the MCDNA nanoparticles exhibited non-cytotoxicity with no obvious inflammation in the transfection sites. These results indicate that stPEI-SPIO nanoparticle is able to serve as both an efficient DNA vector delivery system and a sensitive agent for MRI visualization. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S014296121300149X |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4751]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | BIOMATERIALS |
| 推荐引用方式 GB/T 7714 | Lin Gao,Lisi Xie,Xiaojing Long,et al. Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion[J]. BIOMATERIALS,2013. |
| APA | Lin Gao.,Lisi Xie.,Xiaojing Long.,Zhiyong Wang.,Cheng-Yi He.,...&Bensheng Qiu.(2013).Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion.BIOMATERIALS. |
| MLA | Lin Gao,et al."Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion".BIOMATERIALS (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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