Origin and diversity of novel avian influenza A H7N9 viruses causing human infection:phylogenetic, structural, and coalescent analyses
文献类型：期刊论文
| 作者 | Liu Di; Shi Weifeng; Shi Yi; Wang Dayan; Xiao Haixia; Li Wei; Bi Yuhai; Wu Ying; Li Xianbin; Yan Jinghua |
| 刊名 | LANCET
 |
| 出版日期 | 2013 |
| 英文摘要 | Background On March 30, 2013, a novel avian influenza A H7N9 virus that infects human beings was identified. This virus had been detected in six provinces and municipal cities in China as of April 18, 2013. We correlated genomic sequences from avian influenza viruses with ecological information and didphylogenetic and coalescent analyses to extrapolate the potential origins of the virus and possible routes of reassortment events. Methods We downloaded H7N9 virus genome sequences from the Global Initiative on Sharing Avian Influenza Data (GISAID) database and public sequences used from the Influenza Virus Resource. We constructed phylogenetic trees and did 1000 bootstrap replicates for each tree. Two rounds of phylogeneticanalyses were done. We used at least 100 closely related sequences for each gene to infer the overall topology, removed suspicious sequences from the trees, and focused on the closest clades to the novel H7N9 viruses. We compared our tree topologies with those from a bayesian evolutionary analysis by sampling trees (BEAST) analysis. We used the bayesian Markov chain Monte Carlo method to jointly estimate phylogenies, divergence times, and other evolutionary parameters for all eight gene fragments. We used sequence alignment and homology-modelling methods to study specific mutations regarding phenotypes, specifically addressing the human receptor binding properties. Findings The novel avian influenza A H7N9 virus originated from multiple reassortment events. The HA gene might have originated from avian influenza virusesof duck origin, and the NA gene might have transferred from migratory birds infected with avian influenza viruses along the east Asian flyway. The six internal genes of this virus probably originated from two different groups of H9N2 avian influenza viruses, which were isolated from chickens. Detailed analyses also showed that ducks and chickens probably acted as the intermediate hosts leading to the emergence of this virulent H7N9 virus. Genotypic and potential phenotypic differences imply that the isolates causing this outbreak form two separate subclades. Interpretation The novel avian influenza A H7N9 virus might have evolved from at least four origins. Diversity among isolates implies that the H7N9 virus has evolved into at least two different lineages. Unknown intermediate hosts involved might be implicated, extensive global surveillance is needed, and domestic-poultry-to-person transmission should be closely watched in the future. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0140673613609381 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4784]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | LANCET |
| 推荐引用方式 GB/T 7714 | Liu Di,Shi Weifeng,Shi Yi,et al. Origin and diversity of novel avian influenza A H7N9 viruses causing human infection:phylogenetic, structural, and coalescent analyses[J]. LANCET,2013. |
| APA | Liu Di.,Shi Weifeng.,Shi Yi.,Wang Dayan.,Xiao Haixia.,...&Gao George F..(2013).Origin and diversity of novel avian influenza A H7N9 viruses causing human infection:phylogenetic, structural, and coalescent analyses.LANCET. |
| MLA | Liu Di,et al."Origin and diversity of novel avian influenza A H7N9 viruses causing human infection:phylogenetic, structural, and coalescent analyses".LANCET (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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