MC8 Peptide-Mediated Her-2 Receptor Targeting Based on PEI-β-CyD as Gene Delivery Vector
文献类型：期刊论文
| 作者 | Wei Deng; Hefang Xiao; Xiangfu Zeng; Yiping Hu |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2013 |
| 英文摘要 | A novel vector with high gene delivery efficiency and special cell targeting ability was developed using a good strategy that utilized low molecular weight polyethylenimine (PEI; molecular weight, 600 KDa [PEI600]) cross-linked to β-cyclodextrin (β-CyD) via a facile synthetic route. Human epidermal growth factor receptor 2 (Her-2) are highly expressed in a variety of human cancer cells and are potential targets for cancer therapy. MC8 peptides, which have been proven to combine especially with Her-2 on cell membranes were coupled to PEI-β-CyD using N-succinimidyl-3-(2-pyridyldithio) propionate as a linker. The ratios of PEI600, β-CyD, and peptide were calculated based on proton integral values obtained from the 1H-NMR spectra of the resulting products. Electron microscope observations showed that MC8-PEI-β-CyD can efficiently condense plasmid DNA (pDNA) into nanoparticles of about 200 nm, and MTT assays suggested the decreased toxicity of the polymer. Experiments on gene delivery efficiency in vitro showed that MC8-PEI-β-CyD/pDNA polyplexes had significantly greater transgene activities than PEI-β-CyD/pDNA in the Skov3 and A549 cells, which positively expressed Her-2, whereas, no such effect was observed in the MCF-7 cells, which negatively expressed Her-2. Our current research indicated that the synthesized nonviral vector shows improved gene delivery efficiency and targeting specificity in Her-2 positive cells. |
| 收录类别 | SCI |
| 原文出处 | http://link.springer.com/article/10.1007/s12010-012-9959-2 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4904]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY |
| 推荐引用方式 GB/T 7714 | Wei Deng,Hefang Xiao,Xiangfu Zeng,et al. MC8 Peptide-Mediated Her-2 Receptor Targeting Based on PEI-β-CyD as Gene Delivery Vector[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013. |
| APA | Wei Deng,Hefang Xiao,Xiangfu Zeng,&Yiping Hu.(2013).MC8 Peptide-Mediated Her-2 Receptor Targeting Based on PEI-β-CyD as Gene Delivery Vector.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY. |
| MLA | Wei Deng,et al."MC8 Peptide-Mediated Her-2 Receptor Targeting Based on PEI-β-CyD as Gene Delivery Vector".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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