Puerarin promotes osteogenesis and inhibits adipogenesis in vitro
文献类型：期刊论文
| 作者 | Nan Wang; Xinluan Wang; Wenxiang Cheng; Huijuan Cao; Peng Zhang; Ling Qin |
| 刊名 | CHINESE MEDICINE
 |
| 出版日期 | 2013 |
| 英文摘要 | Background Puerarin (daidzein 8-C-glucoside) has potential on preventing osteoporosis. This study aims to investigate the effects of puerarin on osteogenesis and adipogenesis in vitro. Methods CCK-8 assay, alkaline phosphatase (ALP) activity and Alizarin Red S were used to measure the effects of puerarin on proliferation, osteoblastic differentiation, and mineralization in osteoblast-like MC3T3-E1 cells. The effects of puerarin on adipogenesis were measured by Oil Red O staining and intracellular triglyceride level in preadipocyte 3T3-L1 cells. The mRNA and protein levels of osteogenesis- and adiopogenesis-related factors were detected by qRT-PCR and western blot, respectively. Further, the secreted osteocalcin levels and nuclear translocation of β-catenin were detected by ELISA and immunofluorescence assay, respectively. Results As to osteogenesis, puerarin could stimulate proliferation (1 μM, P = 0.012; 10 μM, P = 0.015; 20 μM, P = 0.050), ALP activity (20 μM, P = 0.008) and calcium nodule formation (20 μM, P = 0.011) in a dose-dependent manner. Puerarin (20 μM) promoted osteocalcin secretion (P = 0.004) and the protein expression of both osteopontin (P = 0.001) and osteoprotegerin (P = 0.003). As to adipogenesis, puerarin suppressed adipocytes formation and intracellular triglyceride level (P = 0.001). In addition, puerarin (20 μM) decreased the mRNA and protein levels of CCAAT/enhancer binding protein α (P = 0.001, P = 0.002), proliferator-activated receptor γ (P = 0.005, P = 0.003), and adipocyte lipid-binding protein 4 (P = 0.001, P = 0.001). Moreover, phosphorylation of AKT1-Ser437 (10 μM, P = 0.003; 20 μM, P = 0.007) and GSK-Ser9 (10 μM, P = 0.005; 20 μM, P = 0.003), and the nuclear translocation of β-catenin (10 μM, P = 0.006; 10 μM, P = 0.002) were increased in 3T3-L1 cells treated by puerarin. Conclusion Puerarin promoted osteogenesis and inhibited adipogenesis in vivo, and Akt/GSK-3β/β-catenin signaling pathway was involved in the suppression of adipogenesis. |
| 收录类别 | SCI |
| 原文出处 | http://www.cmjournal.org/content/8/1/17 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4916]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | CHINESE MEDICINE |
| 推荐引用方式 GB/T 7714 | Nan Wang,Xinluan Wang,Wenxiang Cheng,et al. Puerarin promotes osteogenesis and inhibits adipogenesis in vitro[J]. CHINESE MEDICINE,2013. |
| APA | Nan Wang,Xinluan Wang,Wenxiang Cheng,Huijuan Cao,Peng Zhang,&Ling Qin.(2013).Puerarin promotes osteogenesis and inhibits adipogenesis in vitro.CHINESE MEDICINE. |
| MLA | Nan Wang,et al."Puerarin promotes osteogenesis and inhibits adipogenesis in vitro".CHINESE MEDICINE (2013). |
入库方式： OAI收割
来源：深圳先进技术研究院
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