A Novel Microfluidic Chip for Assessing Dynamic Adhesion Behavior of Cell-Targeting Microbubbles
文献类型：期刊论文
| 作者 | Fei Yan; Xiang Li; Chunxiang Jiang; Qiaofeng Jin; Zidong Zhang; Robin Shandas; Junru Wu; Xin Liu; Hairong Zheng |
| 刊名 | ULTRASOUND IN MEDICINE AND BIOLOGY
 |
| 出版日期 | 2014 |
| 英文摘要 | The primary aim of this study was to develop a microfluidic chip to study the dynamic adhesion behavior of cell-targeted microbubbles. The microfluidic device is composed of polydimethylsiloxane and is fabricated using the soft lithography technique. Each chamber of the microfluidic chip comprises eight U-shaped microsieves, by which various flow velocity distributions are generated. LyP-1-conjugated microbubbles were prepared by coating the surface of the phospholipid shell of microbubbles with LyP-1 peptides via biotin-avidin linkage. Under static conditions, the resulting targeted microbubbles are able to bind onto the surface of cells on incubation with breast cancer cells. Under dynamic fluid conditions, the cell targeting efficiency of the microbubbles was assessed at various flow velocity distributions in a chamber. Accumulation of targeted microbubbles was strongly influenced by flow velocity. Better retention of targeted microbubbles on cell surfaces was achieved at low mean flow velocities (<0.03 cm/s), in agreement with our computer simulation results. In conclusion, our results indicate that the microfluidic system is a useful platform for studying the microbubble-cell adhesive interaction. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0301562913009836 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5752]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | ULTRASOUND IN MEDICINE AND BIOLOGY |
| 推荐引用方式 GB/T 7714 | Fei Yan,Xiang Li,Chunxiang Jiang,et al. A Novel Microfluidic Chip for Assessing Dynamic Adhesion Behavior of Cell-Targeting Microbubbles[J]. ULTRASOUND IN MEDICINE AND BIOLOGY,2014. |
| APA | Fei Yan.,Xiang Li.,Chunxiang Jiang.,Qiaofeng Jin.,Zidong Zhang.,...&Hairong Zheng.(2014).A Novel Microfluidic Chip for Assessing Dynamic Adhesion Behavior of Cell-Targeting Microbubbles.ULTRASOUND IN MEDICINE AND BIOLOGY. |
| MLA | Fei Yan,et al."A Novel Microfluidic Chip for Assessing Dynamic Adhesion Behavior of Cell-Targeting Microbubbles".ULTRASOUND IN MEDICINE AND BIOLOGY (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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