Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome–microbubble complexes assisted by ultrasound
文献类型：期刊论文
| 作者 | Zhiting Deng; Fei Yan; Qiaofeng Jin; Fei Li; Junru Wu; Xin Liu; Hairong Zheng |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2014 |
| 英文摘要 | The circumvention of multidrug resistance (MDR) plays a critically important role in the success of chemotherapy. The aim of this work is to investigate the effectiveness and possible mechanisms of the reversal of MDR phenotype in human breast cancer cells by using doxorubicin-liposome–microbubble complexes (DLMC) assisted by ultrasound (US). DLMC is fabricated through conjugating doxorubicin (DOX)-liposome (DL) to the surface of microbubbles (MBs) via the biotin–avidin linkage. The resulting drug-loaded complexes are then characterized and incubated with MCF-7/ADR human breast cancer cells and followed by US exposure. Our results show the more rapid cellular uptake, evident enhancement of nuclear accumulation and less drug efflux in the resistant cells treated by DLMC + US than those treated by DL, DL + verapamil under the same US treatment or DLMC without US. The enhanced drug delivery and cellular uptake also associated with the increase of cytotoxicity against MCF-7/ADR cells, lower MCF-7/ADR cell viability and higher apoptotic cells. Mechanism investigations further disclose a significant increase of reactive oxygen species (ROS) level, enhanced DNA damage and obvious reduction of P-glycoprotein expression in the resistant cells treated with DLMC + US compared with the control cases of cells treated by DLMC, DL + US or DL + verapamil + US. In conclusion, our study demonstrates that DLMC in combination with US may provide an effective delivery of drug to sensitize cells to circumvent MDR and to enhance the therapeutic index of the chemotherapy. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S016836591300919X |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5753]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | JOURNAL OF CONTROLLED RELEASE |
| 推荐引用方式 GB/T 7714 | Zhiting Deng,Fei Yan,Qiaofeng Jin,et al. Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome–microbubble complexes assisted by ultrasound[J]. JOURNAL OF CONTROLLED RELEASE,2014. |
| APA | Zhiting Deng.,Fei Yan.,Qiaofeng Jin.,Fei Li.,Junru Wu.,...&Hairong Zheng.(2014).Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome–microbubble complexes assisted by ultrasound.JOURNAL OF CONTROLLED RELEASE. |
| MLA | Zhiting Deng,et al."Reversal of multidrug resistance phenotype in human breast cancer cells using doxorubicin-liposome–microbubble complexes assisted by ultrasound".JOURNAL OF CONTROLLED RELEASE (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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