New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy
文献类型：期刊论文
| 作者 | Xiaohong Yang; Ling Qin; Weiguo Liang; Wen Wang; Jianrong Tan; Peihong Liang; Jiake Xu; Siming Li; Shuliang Cui |
| 刊名 | CALCIFIED TISSUE INTERNATIONAL
 |
| 出版日期 | 2014 |
| 英文摘要 | Bone is a mineralized connective tissue that is continuously and microstructurally remodeled. Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone formation and microstructure will provide insight into the understanding of bone pathogenesis and remodeling. Here, new bone formation ex vitro and its microstructure were evaluated in in vivo multiple sequential polychrome-labeled samples using confocal laser scanning microscopy (CLSM), which generated clearer and more reliable images of thick bone sections than conventional fluorescence microscopy (CFM). Intriguingly, fine details of the bone microstructural features, including the mineralization fronts, quiescent versus active osteons, and Volkmann's channel, were elucidated using CLSM, which defines the relationship between morphological changes and function, when combined with differential interference contrast microscopy. Furthermore, CLSM provided objective evaluations of bone formation, such as the ratio of labeled areas of new bone formation in a rabbit model when compared with CFM. Altogether, new bone formation and its microstructure can be evaluated more adequately using a combination of CLSM and DIC microscopies. |
| 收录类别 | SCI |
| 原文出处 | http://download.springer.com/static/pdf/256/art%253A10.1007%252Fs00223-013-9815-6.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00223-013-9815-6&token2=exp=1433671178~acl=%2Fstatic%2Fpdf%2F256%2Fart%25253A10.1007%25252Fs00223-013-9815-6.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs00223-013-9815-6*~hmac=c82a923e240abf0bc6903c5404bc58b26bfa53bf8610c6abc0c51d21b0900331 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5847]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | CALCIFIED TISSUE INTERNATIONAL |
| 推荐引用方式 GB/T 7714 | Xiaohong Yang,Ling Qin,Weiguo Liang,et al. New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy[J]. CALCIFIED TISSUE INTERNATIONAL,2014. |
| APA | Xiaohong Yang.,Ling Qin.,Weiguo Liang.,Wen Wang.,Jianrong Tan.,...&Shuliang Cui.(2014).New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy.CALCIFIED TISSUE INTERNATIONAL. |
| MLA | Xiaohong Yang,et al."New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy".CALCIFIED TISSUE INTERNATIONAL (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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