Occurrence of low frequency PIK3CA and AKT2 mutations in gastric cancer. Mutation Research
文献类型：期刊论文
| 作者 | Qing-Ying Zhanga; Wen-Xiang Cheng; Wen-Mei Li; William Au; You-Yong Lu |
| 刊名 | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
 |
| 出版日期 | 2014 |
| 英文摘要 | The PI3K/AKT signal transduction pathway has distinct functional roles in tumor progression. PIK3CA was reported to harbor the hot-spot in many types of tumor. Akt, the downstream of PI3K, its family members especially AKT2 activation in human cancer has been extensively studied, but its activation by mutation was less reported. The occurrence of PIK3CA and AKT2 mutations in a variety of cancers indicates their important involvement in carcinogenesis. Therefore, we investigated their mutation frequencies in gastric cancer (GC) in China. In our study, we selected hot-spot related exons 9, 18 and 20 of PIK3CA and kinase domain exons 6-14 of AKT2 genes were screened in 10 GC cell lines, 100 advanced primary GC and matched normal tissues. Denaturing high performance liquid chromatography (DHPLC) and DNA sequencing were used to analyze the mutations in the two genes. Two point mutations in the PIK3CA gene were identified in 4 of 10 GC cell lines and in 4 of 100 GC primary tumors. Two polymorphisms in AKT2 were detected in 19 of 100 GC primary tumors. One point mutation in AKT2 was detected in 1 of 10 GC cell lines and 3 of 100 GC primary tumors but no hot spot variation was detected. Our results indicate that PIK3CA and AKT2 mutations occurred at low frequency in GC, and suggest that the PIK3CA/AKT2 pathway might engage other events during gastric carcinogenesis. |
| 收录类别 | SCI |
| 原文出处 | http://ac.els-cdn.com/S0027510714001353/1-s2.0-S0027510714001353-main.pdf?_tid=49cfc060-0cfa-11e5-8e60-00000aab0f01&acdnat=1433670662_a9543a5399c26b161bf6016ea56ee666 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5848]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS |
| 推荐引用方式 GB/T 7714 | Qing-Ying Zhanga,Wen-Xiang Cheng,Wen-Mei Li,et al. Occurrence of low frequency PIK3CA and AKT2 mutations in gastric cancer. Mutation Research[J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS,2014. |
| APA | Qing-Ying Zhanga,Wen-Xiang Cheng,Wen-Mei Li,William Au,&You-Yong Lu.(2014).Occurrence of low frequency PIK3CA and AKT2 mutations in gastric cancer. Mutation Research.MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS. |
| MLA | Qing-Ying Zhanga,et al."Occurrence of low frequency PIK3CA and AKT2 mutations in gastric cancer. Mutation Research".MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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