Callus formation is related to the expression ratios of estrogen receptors-alpha and -beta inovariectomy-induced osteoporotic fracture healing
文献类型：期刊论文
| 作者 | Chow Simon K.; Leung Kwok-sui; Qin Ling; Wei Fangyuan; Cheung Wing-hoi |
| 刊名 | ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
 |
| 出版日期 | 2014 |
| 英文摘要 | This study characterizes ovariectomized (OVX)-induced osteoporotic fracture healing with focus on estrogen receptors (ERs). Callus formation plays a critical role in fracture healing, and ERs are well-known mechanosensors in osteogenic pathways. It was hypothesized that callus formation was related to and partially determined by the difference in expression patterns of ERs in both normal and OVX-induced osteoporotic fractures. Closed femoral fracture in SHAM and ovariectomized rats were used in this study. Weekly callus width (CW) and area (CA), endpoint mechanical properties, gene expressions of Col-1, BMP-2, ER-alpha, ER-beta and ER-alpha:ER-beta ratios (ER-ratios), and correlations were assessed at 2, 4 and 8 weeks post-fracture. CW and CA results confirmed that OVX-induced osteoporotic fracture was delayed at 2-4 weeks with impaired endpoint mechanical properties. Gene expressions of ER-alpha and ER-beta were higher in the SHAM group at week 2 (p < 0.05) and later lowered at week 8; whereas the OVX group showed an opposing trend. Moderate correlation existed between ER-alpha and BMP-2 (0.545, p = 0.003), and ER-ratio and BMP-2 (0.601, p = 0.001), and BMP-2 to CW and CA (r = 0.709, p = 0.000 and r = 0.588, p = 0.001, respectively). ER-alpha and ER-beta proteins expressions were confirmed by immunohistochemistry atthe fracture callus in reparative progenitor cells, osteoblasts- and osteoclasts-like cells. We conclude that the delayed healing rate and impaired callus quality in OVX-induced osteoporotic fracture is related to the delayed expression of ERs. A high ER-alpha:ER-beta ratio favors callus formation. |
| 收录类别 | SCI |
| 原文出处 | http://link.springer.com/article/10.1007%2Fs00402-014-2070-0 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5853]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY |
| 推荐引用方式 GB/T 7714 | Chow Simon K.,Leung Kwok-sui,Qin Ling,et al. Callus formation is related to the expression ratios of estrogen receptors-alpha and -beta inovariectomy-induced osteoporotic fracture healing[J]. ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY,2014. |
| APA | Chow Simon K.,Leung Kwok-sui,Qin Ling,Wei Fangyuan,&Cheung Wing-hoi.(2014).Callus formation is related to the expression ratios of estrogen receptors-alpha and -beta inovariectomy-induced osteoporotic fracture healing.ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY. |
| MLA | Chow Simon K.,et al."Callus formation is related to the expression ratios of estrogen receptors-alpha and -beta inovariectomy-induced osteoporotic fracture healing".ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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