Flavonoids from Herba epimedii selectively activate estrogen receptor alpha (ER alpha) and stimulate ER-dependent osteoblastic functions in UMR-106 cells
文献类型：期刊论文
| 作者 | Xiao Hui-Hui; Fung Chung-Yan; Mok Sao-King; Wong Ka-Chun; Ho Ming-Xian; Wang Xin-Luan; Yao Xin-Sheng; Wong Man-Sau |
| 刊名 | JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
 |
| 出版日期 | 2014 |
| 英文摘要 | Total flavonoids in Herba epimedii (HEP) have been demonstrated to protect against bone loss and bone deterioration associated with estrogen deficiency without exerting any uterotrophic effects. However, it is unclear how flavonoids in HEP exert their protective effects on bone and if different flavonoids exert estrogenic actions in bone cells via similar mechanism of actions. The present study aims to investigate the bone anabolic effects of four major flavonoids isolated from HEP, namely icariin, baohuoside-I, epimedin B and sagittatoside A as well as the mechanism involved in mediating their estrogenic actions in rat osteoblastic-like UMR-106 cells. All tested compounds significantly stimulated the cell proliferation rate, alkaline phosphate (ALP) activity and osteoprotegerin (OPG)/receptor activator of nuclear factor κ-B ligand (RANKL) mRNA expression in UMR-106 cells and their effects could be abolished by co-incubation with 10−6 M ICI 182,780. None of the flavonoids exhibited binding affinities toward ERα and ERβ. However, sagittatoside A selectively activated estrogen response element (ERE)-luciferase activity via ERα. In addition, icariin and sagittatoside A induced ERα phosphorylation at serine 118 residue. Taken together, our results indicated that all four flavonoids from HEP stimulated ER-dependent osteoblastic functions in UMR-106 cells, but only two of them appeared to exert their actions by ligand-independent activation of ERα. Our study provides evidence to support the hypothesis that the estrogen-like protective effects on bone by flavonoids are mediated via mechanisms that are distinct from the classical actions of estrogen. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S096007601400048X# |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/5856]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY |
| 推荐引用方式 GB/T 7714 | Xiao Hui-Hui,Fung Chung-Yan,Mok Sao-King,et al. Flavonoids from Herba epimedii selectively activate estrogen receptor alpha (ER alpha) and stimulate ER-dependent osteoblastic functions in UMR-106 cells[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,2014. |
| APA | Xiao Hui-Hui.,Fung Chung-Yan.,Mok Sao-King.,Wong Ka-Chun.,Ho Ming-Xian.,...&Wong Man-Sau.(2014).Flavonoids from Herba epimedii selectively activate estrogen receptor alpha (ER alpha) and stimulate ER-dependent osteoblastic functions in UMR-106 cells.JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY. |
| MLA | Xiao Hui-Hui,et al."Flavonoids from Herba epimedii selectively activate estrogen receptor alpha (ER alpha) and stimulate ER-dependent osteoblastic functions in UMR-106 cells".JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY (2014). |
入库方式： OAI收割
来源：深圳先进技术研究院
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