Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein
文献类型：期刊论文
| 作者 | Yiseul Ryu; Zongwen Jin; Joong-jae Lee; Seung-hyun Noh; Tae-Hyun Shin; Seong-Min Jo; Joonsung Choi; HyunWook Park; Jinwoo Cheon; and Hak-Sung Kim |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2015 |
| 英文摘要 | Nanoparticle clusters (NPCs) have attracted significant interest owing to their unique characteristics arising from their collective individual properties. Nonetheless, the construction of NPCs in a structurally well-defined and sizecontrollable manner remains a challenge. Here we demonstrate a strategy to construct size-controlled NPCs using the DNAbinding zinc finger (ZnF) protein. Biotinylated ZnF was conjugated to DNA templates with different lengths, followed by incubation with neutravidin-conjugated nanoparticles. The sequence specificity of ZnF and programmable DNA templates enabled a size-controlled construction of NPCs, resulting in a homogeneous size distribution. We demonstrated the utility of magnetic NPCs by showing a three-fold increase in the spin–spin relaxivity in MRI compared with Feridex. Furthermore, folate-conjugated magnetic NPCs exhibited a specific targeting ability for HeLa cells. The present approach can be applicable to other nanoparticles, finding wide applications in many areas such as disease diagnosis, imaging, and delivery of drugs and genes. |
| 收录类别 | SCI |
| 原文出处 | http://onlinelibrary.wiley.com/doi/10.1002/anie.201408593/full |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7039]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION |
| 推荐引用方式 GB/T 7714 | Yiseul Ryu,Zongwen Jin,Joong-jae Lee,et al. Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015. |
| APA | Yiseul Ryu.,Zongwen Jin.,Joong-jae Lee.,Seung-hyun Noh.,Tae-Hyun Shin.,...&and Hak-Sung Kim.(2015).Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. |
| MLA | Yiseul Ryu,et al."Size-Controlled Construction of Magnetic Nanoparticle Clusters Using DNA-Binding Zinc Finger Protein".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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