Longitudinal label-free optical-resolution photoacoustic microscopy of tumor angiogenesis in vivo
文献类型：期刊论文
| 作者 | Riqiang Lin; Jianhua Chen; Huina Wang; Meng Yan; Wei Zheng; Liang Song |
| 刊名 | Quantitative imaging in medicine and surgery
 |
| 出版日期 | 2015 |
| 英文摘要 | Background: Optical-resolution photoacoustic microscopy (OR-PAM) is a high-resolution imaging technology capable of label-free imaging of the morphology and functions of the microvasculature in vivo. Previous studies of angiogenesis by OR-PAM were carried out primarily with transgenic mice and the mouse ear model. While important findings have been generated using this approach, the application of ORPAM to the more widely used subcutaneous dorsal tumor models remains challenging, largely due to the respiratory and cardiac motion artifacts, as well as the protruding tumor contours. Methods and materials: A noninvasive dorsal skin-fold (N-DSF) model, along with adaptive z-scanning and a corresponding experimental protocol, is developed. Mammary carcinoma cells (4T1) were administered subcutaneously to the backs of female BALB/c mice for tumor inoculation. The mice were anesthetized using a mixture of isofluorane and oxygen. Results: In vivo OR-PAM of angiogenesis with subcutaneous dorsal tumor models in mice has been demonstrated. To test the performance of this method, we have monitored the growth of 4T1 mouse mammary carcinoma in BALB/c mice over a period of 9 days. The major features of tumor angiogenesis, including the change of vascular tortuosity, the dilation of vessel diameters, and the increase of blood supply, have been clearly captured with OR-PAM. Conclusions: In combination with N-DSF model, OR-PAM has demonstrated outstanding capacity to provide label-free monitoring of angiogenesis in tumor. Thus, OR-PAM is of great potential to find broad biomedical applications in the pathophysiological studies of tumor and the treatments for anti-angiogenesis. |
| 收录类别 | 其他 |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4312291/pdf/qims-05-01-023.pdf |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7071]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | Quantitative imaging in medicine and surgery |
| 推荐引用方式 GB/T 7714 | Riqiang Lin,Jianhua Chen,Huina Wang,et al. Longitudinal label-free optical-resolution photoacoustic microscopy of tumor angiogenesis in vivo[J]. Quantitative imaging in medicine and surgery,2015. |
| APA | Riqiang Lin,Jianhua Chen,Huina Wang,Meng Yan,Wei Zheng,&Liang Song.(2015).Longitudinal label-free optical-resolution photoacoustic microscopy of tumor angiogenesis in vivo.Quantitative imaging in medicine and surgery. |
| MLA | Riqiang Lin,et al."Longitudinal label-free optical-resolution photoacoustic microscopy of tumor angiogenesis in vivo".Quantitative imaging in medicine and surgery (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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