Quantification of Bound Microbubbles in Ultrasound Molecular Imaging
文献类型：期刊论文
| 作者 | Daeichin Verya; Akkus Zeynettin; Skachkov Ilya; Kooiman Klazina; Needles Andrew; Sluimer Judith; Janssen Ben; Daemen Mat J. A. P.; van der Steen Antonius F. W.; de Jong Nico |
| 刊名 | IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL
 |
| 出版日期 | 2015 |
| 英文摘要 | Molecular markers associated with diseases can be visualized and quantified noninvasively with targeted ultrasound contrast agent (t-UCA) consisting of microbubbles (MBs) that can bind to specific molecular targets. Techniques used for quantifying t-UCA assume that all unbound MBs are taken out of the blood pool few minutes after injection and only MBs bound to the molecular markers remain. However, differences in physiology, diseases, and experimental conditions can increase the longevity of unbound MBs. In such conditions, unbound MBs will falsely be quantified as bound MBs. We have developed a novel technique to distinguish and classify bound from unbound MBs. In the post-processing steps, first, tissue motion was compensated using block-matching (BM) techniques. To preserve only stationary contrast signals, a minimum intensity projection (MinIP) or 20th-percentile intensity projection (PerIP) was applied. The after-flash MinIP or PerIP was subtracted from the before-flash MinIP or PerIP. In this way, tissue artifacts in contrast images were suppressed. In the next step, bound MB candidates were detected. Finally, detected objects were tracked to classify the candidates as unbound or bound MBs based on their displacement. This technique was validated in vitro, followed by two in vivo experiments in mice. Tumors (n = 2) and salivary glands of hypercholesterolemic mice (n = 8) were imaged using a commercially available scanner. Boluses of 100 mu L of a commercially available t-UCA targeted to angiogenesis markers and untargeted control UCA were injected separately. Our results show considerable reduction in misclassification of unbound MBs as bound ones. Using our method, the ratio of bound MBs in salivary gland for images with targeted UCA versus control UCA was improved by up to two times compared with unprocessed images. |
| 收录类别 | SCI |
| 原文出处 | http://www.ncbi.nlm.nih.gov/pubmed/26067053 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7151]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL |
| 推荐引用方式 GB/T 7714 | Daeichin Verya,Akkus Zeynettin,Skachkov Ilya,et al. Quantification of Bound Microbubbles in Ultrasound Molecular Imaging[J]. IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL,2015. |
| APA | Daeichin Verya.,Akkus Zeynettin.,Skachkov Ilya.,Kooiman Klazina.,Needles Andrew.,...&Bosch Johan G..(2015).Quantification of Bound Microbubbles in Ultrasound Molecular Imaging.IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL. |
| MLA | Daeichin Verya,et al."Quantification of Bound Microbubbles in Ultrasound Molecular Imaging".IEEE TRANSACTIONS ON ULTRASONICS FERROELECTRICS AND FREQUENCY CONTROL (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。