Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice
文献类型：期刊论文
| 作者 | Fei Yan; Wei Yang; Xiang Li; Hongmei Liu; Xiang Nan; Lisi Xie; Dongliang Zhou; Guoxi Xie; Junru Wu; Bensheng Qiu |
| 刊名 | BioMed Research International
 |
| 出版日期 | 2015 |
| 英文摘要 | The goal of this study is to investigate the feasibility of using CD81- (Cluster of Differentiation 81 protein-) targeted microparticles of iron oxide (CD81-MPIO) for magnetic resonance imaging (MRI) of the murine atherosclerosis. CD81- MPIO and IgG-(Immunoglobulin G-) MPIO were prepared by covalently conjugating, respectively, with anti-CD81 monoclonal and IgG antibodies to the surface of the tosyl activated MPIO. The relevant binding capability of the MPIO was examined by incubating them with murine bEnd.3 cells stimulated with phenazine methosulfate (PMS) and its effect inshortening T2 relaxation time was also examined. MRI in apolipoprotein E-deficient mice was studied in vivo. Our results show that CD81-MPIO, but not IgG-MPIO, can bind to the PMS-stimulated bEnd.3 cells. The T2 relaxation time was significantly shortened for stimulated bEnd.3 cells when compared with IgG-MPIO. In vivo MRI in apolipoprotein E-deficient mice showed highly conspicuous areas of low signal after CD81- MPIO injection. Quantitative analysis of the area of CD81-MPIO contrast effects showed 8.96- and 6.98-fold increase in comparison with IgG-MPIO or plain MPIO, respectively (P < 0.01). Histological assay confirmed the expression of CD81 and CD81-MPIO binding onto atherosclerotic lesions. In conclusion, CD81- MPIO allows molecular assessment of murine atherosclerotic lesions by magnetic resonance imaging. |
| 收录类别 | SCI |
| 原文出处 | http://www.hindawi.com/journals/bmri/2015/758616/abs/ |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7161]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | BioMed Research International |
| 推荐引用方式 GB/T 7714 | Fei Yan,Wei Yang,Xiang Li,et al. Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice[J]. BioMed Research International,2015. |
| APA | Fei Yan.,Wei Yang.,Xiang Li.,Hongmei Liu.,Xiang Nan.,...&Hairong Zheng.(2015).Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice.BioMed Research International. |
| MLA | Fei Yan,et al."Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice".BioMed Research International (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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