Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair
文献类型：期刊论文
| 作者 | Ting-Wu Qina; Yu-Long Sun; Andrew R. Thoresona; Scott P. Steinmanna; Peter C. Amadioa; Kai-Nan Ana; Chunfeng Zhao |
| 刊名 | Biomaterials
 |
| 出版日期 | 2015 |
| 英文摘要 | Cell-based tissue engineered tendons have potential to improve clinical outcomes following rotator cuff repair, especially in large or massive rotator cuff tears, which pose a great clinical challenge. The aim of this study was to develop a method of constructing a functional engineered tendon patch for rotator cuff repair with cyclic mechanical stimulation. Decellularized tendon slices (DTSs) were seeded with BMSCs and subjected to cyclic stretching for 1, 3, or 7 days. The mechanical properties, morphologic characteristics and tendon-related gene expression of the constructs were investigated. Viable BMSCs were observed on the DTS after 7 days. BMSCs penetrated into the DTSs and formed dense cell sheets after 7 days of mechanical stretching. Gene expression of type I collagen, decorin, and tenomodulin significantly increased in cyclically stretched BMSC-DTS constructs compared with the unstrained control group (P < 0.05). The ultimate tensile strength and stiffness of the cyclically stretched tendon constructs were similar to the unstrained control group (P > 0.05). In conclusion, mechanical stimulation of BMSC-DTS constructs upregulated expression of tendon-related proteins, promoted cell tenogenic differentiation, facilitated cell infiltration and formation of cell sheets, and retained mechanical properties. The patch could be used as a graft to enhance the surgical repair of rotator cuff tears. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0142961215000873 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7184]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | Biomaterials |
| 推荐引用方式 GB/T 7714 | Ting-Wu Qina,Yu-Long Sun,Andrew R. Thoresona,et al. Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair[J]. Biomaterials,2015. |
| APA | Ting-Wu Qina.,Yu-Long Sun.,Andrew R. Thoresona.,Scott P. Steinmanna.,Peter C. Amadioa.,...&Chunfeng Zhao.(2015).Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair.Biomaterials. |
| MLA | Ting-Wu Qina,et al."Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair".Biomaterials (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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