In vitro and in vivo evaluation of bone morphogenetic protein-2 (BMP-2) immobilized collagen-coated polyetheretherketone (PEEK)
文献类型：期刊论文
| 作者 | Du YaWei; Zhang LiNan; Ye Xin; Nie HeMin; Hou ZengTao; Zeng TengHui; Yan GuoPing; Shang Peng |
| 刊名 | FRONTIERS OF MATERIALS SCIENCE
 |
| 出版日期 | 2015 |
| 英文摘要 | Polyetheretherketone (PEEK) is regarded as one of the most potential candidates of biomaterials in spinal implant applications. However, as a bioinert material, PEEK plays a limited role in osteoconduction and osseointegration. In this study, recombinant human bone morphogenetic protein-2 (rhBMP-2) was immobilized onto the surface of collagen-coated PEEK in order to prepare a multi-functional material. After adsorbed onto the PEEK surface by hydrophobic interaction, collagen was cross-linked with N-(3-dimethylaminopropyl)-N'-ethyl carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). EDC/NHS system also contributed to the immobilization of rhBMP-2. Water contact angle tests, XPS and SEM clearly demonstrated the surface changes. ELISA tests quantified the amount of rhBMP-2 immobilized and the release over a period of 30 d. In vitro evaluation proved that the osteogenesis differentiation rate was higher when cells were cultured on modified PEEK discs than on regular ones. In vivo tests were conducted and positive changes of major parameters were presented. This report demonstrates that the rhBMP-2 immobilized method for PEEK modification increase bioactivity in vitro and in vivo, suggesting its practicability in orthopedic and spinal clinical applications. |
| 收录类别 | SCI |
| 原文出处 | http://link.springer.com/article/10.1007/s11706-015-0276-x |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/7191]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | FRONTIERS OF MATERIALS SCIENCE |
| 推荐引用方式 GB/T 7714 | Du YaWei,Zhang LiNan,Ye Xin,et al. In vitro and in vivo evaluation of bone morphogenetic protein-2 (BMP-2) immobilized collagen-coated polyetheretherketone (PEEK)[J]. FRONTIERS OF MATERIALS SCIENCE,2015. |
| APA | Du YaWei.,Zhang LiNan.,Ye Xin.,Nie HeMin.,Hou ZengTao.,...&Shang Peng.(2015).In vitro and in vivo evaluation of bone morphogenetic protein-2 (BMP-2) immobilized collagen-coated polyetheretherketone (PEEK).FRONTIERS OF MATERIALS SCIENCE. |
| MLA | Du YaWei,et al."In vitro and in vivo evaluation of bone morphogenetic protein-2 (BMP-2) immobilized collagen-coated polyetheretherketone (PEEK)".FRONTIERS OF MATERIALS SCIENCE (2015). |
入库方式： OAI收割
来源：深圳先进技术研究院
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