A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro.
文献类型：期刊论文
| 作者 | Xu, Jinfeng; Zeng, Xinxin; Liu, Yingying; Luo, Hui; Wei, Zhanghong; Liu, Huiyu; Zhou, Yuli; Zheng, Hairong; Zhou, Jie; Tan, Guanghong |
| 刊名 | TUMOR BIOLOGY
 |
| 出版日期 | 2016 |
| 英文摘要 | Ultrasound-targeted microbubble destruction (UTMD) has become a novel gene/drug delivery method in cancer therapeutic application. However, the gene transfection efficiency mediated by UTMD is still unsatisfactory. Here, we introduced iRGD/CCR2 dual-targeted cationic microbubbles (MBiRGD/CCR2) which was modified with PEI-600 and coated with iRGD peptides and anti-CCR-2 antibodies. It showed that MBiRGD/CCR2 had a 25.83 +/- 1.57 mV surface zeta potential and good stability. The experiments in vitro showed MBiRGD/CCR2 had higher binding efficiency with both bEnd.3 cells and MCF-7 cells than that of iRGD or CCR2 single-targeted cationic microbubbles (MBiRGD or MBCCR2) (P < 0.05 for both). Agarose gel electrophoresis assay showed that MBiRGD/CCR2 can effectively load pGPU6/GFP/Neo-shAKT2 plasmid DNA. Compared with the plain MBs (MBcontrol) or single-targeted cationic MBs including MBiRGD and MBCCR2 (P < 0.05 for all), the dual-targeted cationic MBiRGD/CCR2 groups had higher gene transfection efficiency under US exposure. It showed that the dual-targeted cationic MBiRGD/CCR2 has a potential value to be used as an ultrasound imaging probe for ultrasound image-guided tumor gene therapy. |
| 收录类别 | SCI |
| 原文出处 | http://www.sciencedirect.com/science/article/pii/S0911604415300233 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10480]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | TUMOR BIOLOGY |
| 推荐引用方式 GB/T 7714 | Xu, Jinfeng,Zeng, Xinxin,Liu, Yingying,et al. A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro.[J]. TUMOR BIOLOGY,2016. |
| APA | Xu, Jinfeng.,Zeng, Xinxin.,Liu, Yingying.,Luo, Hui.,Wei, Zhanghong.,...&Yan, Fei.(2016).A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro..TUMOR BIOLOGY. |
| MLA | Xu, Jinfeng,et al."A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro.".TUMOR BIOLOGY (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
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