A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo
文献类型：期刊论文
| 作者 | J. Chu; Y. Oh; A. Sens; N. Ataie; H. Dana; J. J. Macklin; T. Laviv; E. S. Welf; K. M. Dean; F. Zhang |
| 刊名 | Nature Biotechnology
 |
| 出版日期 | 2016 |
| 英文摘要 | Orange-red fluorescent proteins (FPs) are widely used in biomedical research for multiplexed epifluorescence microscopy with GFP-based probes, but their different excitation requirements make multiplexing with new advanced microscopy methods difficult. Separately, orange-red FPs are useful for deep-tissue imaging in mammals owing to the relative tissue transmissibility of orange-red light, but their dependence on illumination limits their sensitivity as reporters in deep tissues. Here we describe CyOFP1, a bright, engineered, orange-red FP that is excitable by cyan light. We show that CyOFP1 enables single-excitation multiplexed imaging with GFP-based probes in single-photon and two-photon microscopy, including time-lapse imaging in light-sheet systems. CyOFP1 also serves as an efficient acceptor for resonance energy transfer from the highly catalytic blue-emitting luciferase NanoLuc. An optimized fusion of CyOFP1 and NanoLuc, called Antares, functions as a highly sensitive bioluminescent reporter in vivo, producing substantially brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins. |
| 收录类别 | SCI |
| 原文出处 | http://www.nature.com/nbt/journal/v34/n7/full/nbt.3550.html |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/10503]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 作者单位 | Nature Biotechnology |
| 推荐引用方式 GB/T 7714 | J. Chu,Y. Oh,A. Sens,et al. A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo[J]. Nature Biotechnology,2016. |
| APA | J. Chu.,Y. Oh.,A. Sens.,N. Ataie.,H. Dana.,...&M. Lin.(2016).A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo.Nature Biotechnology. |
| MLA | J. Chu,et al."A bright cyan-excitable orange fluorescent protein facilitates dual-emission microscopy and enhances bioluminescence imaging in vivo".Nature Biotechnology (2016). |
入库方式： OAI收割
来源：深圳先进技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。