The role of surface charge density in cationic liposome-promoted dendritic cell maturation and vaccine-induced immune responses
文献类型：期刊论文
| 作者 | Ma Yifan; Zhuang Yan; Xie Xiaofang; Wang Ce; Wang Fei; Zhou Dongmei; Zeng Jianqiang; Cai Lintao |
| 刊名 | Nanoscale
 |
| 出版日期 | 2011 |
| 卷号 | 3期号:5页码:2307-2314 |
| 英文摘要 | Cationic liposomes have emerged as a novel adjuvant and antigen delivery system to enhance vaccine efficacy. However, the role of surface charge density incationic liposome-regulated immune responses has not yet been elucidated. In the present study, we prepared a series of DOTAP/DOPC cationic liposomes with different surface densities by incorporating varying amounts of DOPC (a neutral lipid) into DOTAP (a cationic lipid). The results showed that DOTAP/DOPCcationic liposome-regulated immune responses relied on the surface charge density, and might occur through ROS signaling. The liposomes with a relatively high charge density, such as DOTAP/DOPC 5 : 0 and 4 : 1 liposomes, potently enhanced dendritic cell maturation, ROS generaion, antigen uptake, as well asthe production of OVA-specific IgG2a and IFN-gamma. In contrast, low-charge liposomes, such as DOTAP/DOPC 1 : 4 liposome, failed to promote immuneresponses even at high concentrations, confirming that the immunoregulatory effect of cationic liposomes is mostly attributable to their surface charge density. Moreover, the DOTAP/DOPC 1 : 4 liposome suppressed anti-OVA antibody responses in vivo. Overall, maintaining an appropriate surface charge is crucial for optimizing the adjuvant effect of cationic liposomes and enhancing the efficacy of liposome-based vaccines. |
| 收录类别 | SCI |
| 原文出处 | http://pubs.rsc.org/en/content/articlepdf/2011/nr/c1nr10166h |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/3647]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | Nanoscale |
| 推荐引用方式 GB/T 7714 | Ma Yifan,Zhuang Yan,Xie Xiaofang,et al. The role of surface charge density in cationic liposome-promoted dendritic cell maturation and vaccine-induced immune responses[J]. Nanoscale,2011,3(5):2307-2314. |
| APA | Ma Yifan.,Zhuang Yan.,Xie Xiaofang.,Wang Ce.,Wang Fei.,...&Cai Lintao.(2011).The role of surface charge density in cationic liposome-promoted dendritic cell maturation and vaccine-induced immune responses.Nanoscale,3(5),2307-2314. |
| MLA | Ma Yifan,et al."The role of surface charge density in cationic liposome-promoted dendritic cell maturation and vaccine-induced immune responses".Nanoscale 3.5(2011):2307-2314. |
入库方式： OAI收割
来源：深圳先进技术研究院
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