Relatively low level of antigen-specific monocytes detected in blood from untreated tuberculosis patients using CD4+ T-cell receptor tetramers
文献类型：期刊论文
| 作者 | Huang, Yuhong; Huang, Yan; Fang, Yimin; Wang, Juan; Li, Yan; Wang, Nan; Zhang, Jianbo; Gao, Ming; Huang, Lirong; Yang, FangfangWang, Cong |
| 刊名 | PLOS PATHOGENS
 |
| 出版日期 | 2012 |
| 英文摘要 | The in vivo kinetics of antigen-presenting cells (APCs) in patients with advanced and convalescent tuberculosis (TB) is not well characterized. In order to target Mycobacterium tuberculosis (MTB) peptides-and HLA-DR-holding monocytes and macrophages, 2 MTB peptide-specific CD4(+) T-cell receptor (TCR) tetramers eu and hu were successfully constructed. Peripheral blood (PBL) samples from inpatients with advanced pulmonary TB (PTB) were analyzed usingflow cytometry, and the percentages of tetramer-bound CD14(+) monocytes ranged from 0.26-1.44% and 0.21-0.95%, respectively; significantly higher than those measured in PBL samples obtained from non-TB patients, healthy donors, and umbilical cords. These tetramers were also able to specifically detect macrophages in situ via immunofluorescent staining. The results of the continuous time-point tracking of the tetramer-positive rates in PBL samples from active PTB outpatients undergoing treatment show that the median percentages were at first low before treatment, increased to their highest levels during the first month, and then began to decrease during the second month until finally reaching and maintaining a relatively low level after 3-6 months. These results suggest that there is a relatively low level of MTB-specific monocytes in advanced and untreated patients. Further experiments show that MTB induces apoptosis inCD14(+) cells, and the percentage of apoptotic monocytes dramatically decreases after treatment. Therefore, the relatively low level of MTB-specificmonocytes is probably related to the apoptosis or necrosis of APCs due to live bacteria and their growth. The bactericidal effects of anti-TB drugs, as well as other unknown factors, would induce a peak value during the first month of treatment, and a relatively low level would be subsequently reached and maintained until all of the involved factors reached equilibrium. These tetramers have diagnostic potential and can provide valuable insights into the mechanisms of antigenpresentation and its relationship with TB infection and latent TB infection. |
| 收录类别 | SCI |
| 原文出处 | http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003036 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/4270]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 作者单位 | PLOS PATHOGENS |
| 推荐引用方式 GB/T 7714 | Huang, Yuhong,Huang, Yan,Fang, Yimin,et al. Relatively low level of antigen-specific monocytes detected in blood from untreated tuberculosis patients using CD4+ T-cell receptor tetramers[J]. PLOS PATHOGENS,2012. |
| APA | Huang, Yuhong.,Huang, Yan.,Fang, Yimin.,Wang, Juan.,Li, Yan.,...&Lai, Xiaomin.(2012).Relatively low level of antigen-specific monocytes detected in blood from untreated tuberculosis patients using CD4+ T-cell receptor tetramers.PLOS PATHOGENS. |
| MLA | Huang, Yuhong,et al."Relatively low level of antigen-specific monocytes detected in blood from untreated tuberculosis patients using CD4+ T-cell receptor tetramers".PLOS PATHOGENS (2012). |
入库方式： OAI收割
来源：深圳先进技术研究院
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