中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification

文献类型:期刊论文

作者Xu, Qinfeng; Cao, Anping; Zhang, Ling-fei; Zhang, Chun-yang
刊名ANALYTICAL CHEMISTRY
出版日期2012
英文摘要Target recycling-oriented amplification has been widely applied for sensitive detection of DNA, RNA, and proteins due to its successful overcoming the inherent limitation of target-to-signal ratio of 1:1 in the traditional hybridization assay. Exonuclease III (Exo III) is usually used as the cleavage enzyme in the targetrecycling-oriented amplification because of its easy availability, high catalytic activity, and wide applicability. Even though Exo III is assumed to be double-stranded DNA (dsDNA) specific exonuclease in most literature, its cleavage of single-strand DNA (ssDNA) does occur, resulting in the target-independent degradation of probes. Herein, we design an intramolecular displacement probe with the capability of resistance to the nonspecific digestion of Exo III and fast hybridization kinetics. Through the substitution of 2-aminopurine for adenine in the intramolecular displacement probes, we develop a rapid and label-freeapproach to monitor Exo III-assisted target recycling amplification. We further demonstrate that this method can be used for the detection of DNA and proteins with excellent specificity and high sensitivity. Importantly, this method can be extended to rapid, label-free and multiplexed detection of various nucleic acids, proteins, and small molecules using different kinds of fluorescent nucleotide analogues and specific aptamers.
收录类别SCI
原文出处http://pubs.acs.org/doi/abs/10.1021/ac303095z
语种英语
源URL[http://ir.siat.ac.cn:8080/handle/172644/4280]  
专题深圳先进技术研究院_医药所
作者单位ANALYTICAL CHEMISTRY
推荐引用方式
GB/T 7714
Xu, Qinfeng,Cao, Anping,Zhang, Ling-fei,et al. Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification[J]. ANALYTICAL CHEMISTRY,2012.
APA Xu, Qinfeng,Cao, Anping,Zhang, Ling-fei,&Zhang, Chun-yang.(2012).Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification.ANALYTICAL CHEMISTRY.
MLA Xu, Qinfeng,et al."Rapid and label-free monitoring of exonuclease III-assisted target recycling amplification".ANALYTICAL CHEMISTRY (2012).

入库方式: OAI收割

来源:深圳先进技术研究院

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